| 项目编号: | BB/K01613X/1
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| 项目名称: | Molecular and genetic networks determining row number in cultivated barley. |
| 作者: | Robbie Waugh
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| 承担单位: | The James Hutton Institute
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| 批准年: | 2013
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| 开始日期: | 2014-03-02
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| 结束日期: | 2017-02-02
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| 资助金额: | GBP363679
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| 资助来源: | UK-BBSRC
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| 项目类别: | Research Grant
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| 国家: | UK
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| 语种: | 英语
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| 特色学科分类: | Agri-environmental science
; Plant & crop science
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| 英文摘要: | Wild barleys, the original domesticated forms and the majority of current elite UK cultivars produce two rows of grain bearing spikelets either side of the inflorescence, or spike. However, soon after the domestication, barleys with six rows of grain emerged that ultimately dominated early barley cultivation. Mutations in a single gene called SIX-ROWED SPIKE 1 (VRS1) have been identified as responsible for this important developmental switch. In two-rowed types, VRS1 mRNA is expressed in progenitor cells of the lateral spikelets which remain sterile presumably because VRS1 protein represses expression of genes that are required for development of 'lateral fertility'. Inactivating VRS1 via mutation would de-repress expression of lateral fertility genes, resulting in a six-rowed spike. While VRS1 is core to this process, we also know from historical studies with barley mutants that at least 11 different SIX-ROWED SPIKE genes influence the degree of fertility of the lateral spikelets. For example, we recently identified SIX-ROWED SPIKE 5 (VRS5), and showed that different versions of this gene (that we call 'alleles') are always paired with different versions of VRS1 in commercial two- and six- rowed barleys. This pairing is important because in lines that have the six-row version of VRS1 (denoted as vrs1), a two-row VRS5 allele (Vrs5) causes the development of small grain from the lateral spikelets. In contrast, the six-row version of VRS5 (vrs5) causes the lateral spikelets to develop fully, with important consequences on yield. This observation demonstrates that getting the correct combination of alleles at VRS genes is extremely important. While mutant studies have identified many VRS genes, we recently showed that natural variation in only four genes is associated with determining whether current elite UK barley cultivars are genetically optimal two- or six-row-types. As expected, one of these was VRS1 and another VRS5. We recently identified the third gene as VRS3 and are trying to identify the fourth, which does not coincide with the location of any of the eleven VRS mutants. In parallel, VRS4 has been identified by German collaborators.
While we now know these genes are intrinsically linked by their involvement in the developmental pathway that restores fertility to a nascent floral organ (i.e. the lateral spikelets) at the moment we have no idea if or how these components interact, what other genes/proteins are involved or how six-rowed types evolved over the 10,000 years since the domestication of the species. These are the issues we plan to address in this project. We believe that a better understanding of this fundamental developmental process will provide insights into how we can exploit variation in genes controlling plant morphology and architecture to ultimately improve plant yield. |
| 资源类型: | 项目
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| 标识符: | http://119.78.100.158/handle/2HF3EXSE/101870
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| Appears in Collections: | 科学计划与规划
气候变化与战略
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作者单位: | The James Hutton Institute
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| Recommended Citation: | |
Robbie Waugh. Molecular and genetic networks determining row number in cultivated barley.. 2013-01-01.
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