DOI: 10.1016/j.jcou.2018.09.020
Scopus记录号: 2-s2.0-85054258050
论文题名: Enzymatic CO2 reduction to formate by formate dehydrogenase from Candida boidinii coupling with direct electrochemical regeneration of NADH
作者: Barin R. ; Biria D. ; Rashid-Nadimi S. ; Asadollahi M.A.
刊名: Journal of CO2 Utilization
ISSN: 22129820
出版年: 2018
卷: 28 起始页码: 117
结束页码: 125
语种: 英语
英文关键词: CO2 reduction
; Electrospun nanofiber
; Formate dehydrogenase
; Immobilization
; NADH regeneration
Scopus关键词: Carbon dioxide
; Electrochemistry
; Enzyme activity
; Nanofibers
; Radioactive waste vitrification
; Reduction
; Reusability
; CO2 reduction
; Electrochemical regeneration
; Electrospun nanofibers
; Enzymatic conversions
; Formate dehydrogenase
; Long-term storage stability
; NADH regeneration
; Polystyrene nanofibers
; Copper compounds
英文摘要: Enzymatic conversion of CO2 to formate was carried out in the cathodic cell of a two-chamber electrochemical apparatus where NAD+ was reduced on the surface of a Copper foam electrode. Formate dehydrogenase (FDH) was used as the biocatalyst in both free form and immobilized on the modified electrospun polystyrene nanofibers (EPSNF). The fabricated EPSNF were modified by a multistage procedure including acid treatment, silanization followed by activation with glutaraldehyde. The effects of regenerated NADH concentration and time of enzymatic reaction on the formate production in the both systems were studied. The results indicated that the EPSNF immobilized FDH had a desirable activity, long-term storage stability (41% after 20 days) and reusability after eight cycles of successive reactions (53% of the initial activity). Moreover, it was revealed that the increase of cofactor concentration at the early times of reaction was favorable to the formate production. However, an inhibitory effect was observed at higher concentrations of NADH, and the optimum values of 0.45 mM and 0.51 mM were obtained for the maximum enzyme activity by the free and immobilized enzymes respectively. The produced formate at the optimum cofactor concentration after 300 min was 0.61 mM and 0.31 mM for the free and immobilized enzyme systems. Finally, it can be concluded that the presented process is a promising approach to the enzymatic conversion of CO2. © 2018 Elsevier Ltd. All rights reserved.. Citation statistics:
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/111754
Appears in Collections: 气候减缓与适应
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作者单位: Department of Biotechnology, Faculty of Advanced Sciences and Technologies, University of Isfahan, Iran; Department of Renewable Energy Engineering, Faculty of Advanced Sciences and Technologies, University of Isfahan, Iran
Recommended Citation:
Barin R.,Biria D.,Rashid-Nadimi S.,et al. Enzymatic CO2 reduction to formate by formate dehydrogenase from Candida boidinii coupling with direct electrochemical regeneration of NADH[J]. Journal of CO2 Utilization,2018-01-01,28