DOI: 10.1016/j.jcou.2018.08.010
Scopus记录号: 2-s2.0-85052834683
论文题名: Carbon dioxide biofixation by free air CO2 enriched (FACE) bacterium for biodiesel production
作者: Maheshwari N. ; Kumar M. ; Thakur I.S. ; Srivastava S.
刊名: Journal of CO2 Utilization
ISSN: 22129820
出版年: 2018
卷: 27 起始页码: 423
结束页码: 432
语种: 英语
英文关键词: Bacillus sp. SS105
; Carbonic anhydrase
; CO2 sequestration
; Fatty acid methyl esters
; Ribulose 1-5 bisphosphate carboxylase/oxygenase
Scopus关键词: Bacteria
; Bacteriology
; Biodiesel
; Carbonic anhydrase
; Electrophoresis
; Esters
; Genes
; Palmitic acid
; Unsaturated fatty acids
; Bacillus sp
; Bisphosphates
; Carbon dioxide sequestration
; Carbon-concentrating mechanism
; CO2 sequestration
; Fatty acid methyl ester
; Fatty acid methyl esters (FAMEs)
; Western-blot analysis
; Carbon dioxide
英文摘要: Microbial CO2 fixation is an emerging strategy to mitigate CO2 emissions and its transformation into value added products by exploring potential bacteria. In this study, bacterial strains were isolated from Free Air CO2 Enrichment (FACE) soil area and screened for carbon dioxide sequestration and lipid production. CO2 sequestration efficiency of bacterial strains was determined via enzymatic activity of carbonic anhydrase (CA) and ribulose-1, 5-bisphosphate carboxylase/oxygenase (RuBisCO) involved in carbon concentrating mechanism (CCM). Bacillus sp. SS105 was found to be the most efficient strain for CCM and showed maximum biomass productivity (381 mg l-1d-1), and CO2 consumption rate (287.21 mg l-1d-1). Western blot analysis confirmed the presence of RuBisCO large subunit in Bacillus sp. SS105. Further genes were amplified from SS105 genome using degenerate primers. Amplified gene sequences showed homology with CA and rbcL gene. Bacterium SS105 has shown higher lipid production as compared to other strains. The lipid yield increased 1.5-fold after optimization. The chemical structure and composition of Fatty acid methyl esters (FAMEs) were characterized by GC - MS, FTIR and 1H NMR. Total FAMEs content was quantified as 120 mg l-1 and showed 51 % of saturated and 49% of unsaturated fatty acids. Among the FAMEs, palmitic (37.80 %) and oleic acid (37.88 %) were present in high proportion which is a prerequisite for good quality biodiesel. The study highlights the importance of Bacillus sp. SS105 in CO2sequestration and biodiesel production with potential future applications. © 2018 Elsevier Ltd. Citation statistics:
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/111813
Appears in Collections: 气候减缓与适应
There are no files associated with this item.
作者单位: Amity School of Earth and Environmental Science, Amity University, Gurugram, Haryana, India; School of Environmental Sciences, Jawaharlal Nehru University, New Delhi, 110067, India
Recommended Citation:
Maheshwari N.,Kumar M.,Thakur I.S.,et al. Carbon dioxide biofixation by free air CO2 enriched (FACE) bacterium for biodiesel production[J]. Journal of CO2 Utilization,2018-01-01,27