DOI: 10.1016/j.jcou.2018.08.014
Scopus记录号: 2-s2.0-85052516798
论文题名: Supercritical CO2 extraction of an immunosuppressant produced by solid-state fermentation
作者: Alpak I. ; Askin Uzel R. ; Sargin S. ; Yesil-Celiktas O.
刊名: Journal of CO2 Utilization
ISSN: 22129820
出版年: 2018
卷: 27 起始页码: 398
结束页码: 404
语种: 英语
英文关键词: Bioreactor
; Immunosuppressant
; Mycophenolic acid
; Scale-up
; Supercritical CO2
Scopus关键词: Bioconversion
; Bioreactors
; Enzyme activity
; Ethanol
; Fermentation
; Metabolites
; Mixtures
; Organic solvents
; Substrates
; Different carbon sources
; Immunosuppressant
; Mycophenolic acids
; Penicillium brevicompactum
; Scale-up
; Solid-state fermentation
; Supercritical CO2
; Supercritical CO2 extraction
; Carbon dioxide
英文摘要: Mycophenolic acid (MPA) is a secondary metabolite of Penicillium species with diverse biological properties and has clinical applications mainly as an immunosuppressive agent. Production of MPA was carried out using Penicillium brevicompactum (DSM 2215) by solid-state fermentation (SSF). Six different carbon sources (wheat bran, rice husk, rice bran, potato peel and rice husk - rice bran, rice bran-potato peel mixture) were examined by SSF in order to obtain the highest yield of mycophenolic acid. Rice bran-potato peel mixture was determined as the best solid substrate and used in scale up studies which was applied in the tray bioreactor. Supercritical CO2 and soxhlet extractions were analyzed for the recovery of MPA from the fermentation broth. The highest recovery (0.47 g MPA/100 g substrate) was obtained by supercritical CO2 at 30 MPa, 50 °C at a CO2 flow rate of 15 g/min with 10% ethanol as co-solvent for 3 h. The results obtained by the scale-up in bioreactor and optimization of supercritical CO2 entrained with ethanol can provide basis for the production of the immunosuppresant compound on industrial scale. © 2018 Elsevier Ltd. All rights reserved. Citation statistics:
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/111816
Appears in Collections: 气候减缓与适应
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作者单位: Department of Bioengineering, Faculty of Engineering, Ege University, Bornova-Izmir, 35100, Turkey; Department of Food Processing, Yasar University, Bornova-Izmir, 35100, Turkey
Recommended Citation:
Alpak I.,Askin Uzel R.,Sargin S.,et al. Supercritical CO2 extraction of an immunosuppressant produced by solid-state fermentation[J]. Journal of CO2 Utilization,2018-01-01,27