DOI: 10.1016/j.jcou.2018.01.018
Scopus记录号: 2-s2.0-85041466555
论文题名: Investigation on CO2 bio-mitigation using Halomonas stevensii in laboratory scale bioreactor: Design of downstream process and its economic feasibility analysis
作者: Mishra S. ; Pahari S. ; Siva K. ; Mohanty S. ; Gupta S. ; Raghuvanshi S.
刊名: Journal of CO2 Utilization
ISSN: 22129820
出版年: 2018
卷: 24 起始页码: 274
结束页码: 286
语种: 英语
英文关键词: CO2 bio-mitigation
; Downstream bio-processing
; Halomonas stevensii
; Process economics
; Thermodynamic analysis
Scopus关键词: Alcohols
; Carbon
; Carbon dioxide
; Fourier transform infrared spectroscopy
; Gas chromatography
; Mass spectrometry
; Metabolites
; Molecular biology
; Solvent extraction
; Thermoanalysis
; Thermodynamic properties
; CO2 bio-mitigation
; Downstream-processing
; Economic feasibilities
; Economic feasibility analysis
; Halomonas
; Process economics
; Processing strategies
; Thermo dynamic analysis
; Economic analysis
英文摘要: In the present study, H. stevensii was cultivated on a semi-continuous mode in a laboratory scale bio-reactor using CO2(g) [15% (v/v)] as carbon source and thiosulfate (S2O3 2−) as an energy source for the total duration of three days. Approximately, 100% CO2(g) removal from gaseous phase was achieved. Leachate obtained was subjected to different downstream bio-processing strategies. Biomass harvesting using filtration and recovery of metabolites without cell disruption using solvent extraction from wet biomass was observed as the best downstream processing strategy. Qualitative analysis of products was carried out using gas chromatography and mass spectroscopy (GC–MS) and their results have indicated fatty alcohols (C8–C27) as primary metabolites. Fourier transform infrared spectroscopy (FTIR) analysis, approximate material balance and thermodynamic analysis have confirmed the intracellular assimilation of CO2(g) as HCO3 − and its metabolization into fatty alcohols. Economic feasibility of the process has suggested that the developed downstream process has the capability to replace coconut oil based process for the production of fatty alcohols (C12–C14) and it can be utilized for the production of dodecanol as compared to the tetradecanol. © 2018 Elsevier Ltd Citation statistics:
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/111962
Appears in Collections: 气候减缓与适应
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作者单位: Department of Chemical Engineering, Birla Institute of Technology and Science (BITS), Pilani, Rajasthan 333031, India
Recommended Citation:
Mishra S.,Pahari S.,Siva K.,et al. Investigation on CO2 bio-mitigation using Halomonas stevensii in laboratory scale bioreactor: Design of downstream process and its economic feasibility analysis[J]. Journal of CO2 Utilization,2018-01-01,24