DOI: 10.1016/j.watres.2018.08.028
Scopus记录号: 2-s2.0-85051935013
论文题名: Assessing the impact of wastewater treatment plant effluent on downstream drinking water-source quality using a zebrafish (Danio Rerio) liver cell-based metabolomics approach
作者: Zhen H. ; Ekman D.R. ; Collette T.W. ; Glassmeyer S.T. ; Mills M.A. ; Furlong E.T. ; Kolpin D.W. ; Teng Q.
刊名: Water Research
ISSN: 431354
出版年: 2018
卷: 145 起始页码: 198
结束页码: 209
语种: 英语
英文关键词: Cell culture
; Metabolomics
; Potable reuse
; Wastewater treatment plant effluent
; Water quality
Scopus关键词: Biological water treatment
; Cell culture
; Cells
; Discharge (fluid mechanics)
; Effluent treatment
; Effluents
; Gas chromatography
; Hydrophilicity
; Inlet flow
; Location
; Mass spectrometry
; Metabolism
; Metabolites
; Multivariant analysis
; Nuclear magnetic resonance
; Nuclear magnetic resonance spectroscopy
; Outfalls
; Physiology
; Potable water
; Sewage pumping plants
; Wastewater reclamation
; Wastewater treatment
; Water conservation
; Water quality
; Water treatment plants
; Drinking water treatment process
; Gas chromatography-mass spectrometries (GC-MS)
; Metabolomics
; Multivariate statistical analysis
; Potable reuse
; Proton nuclear magnetic resonance
; Wastewater treatment plant effluent
; Wastewater treatment plants
; Rivers
; cell extract
; drinking water
; cell
; cyprinid
; discharge
; distribution system
; effluent
; measurement method
; nuclear magnetic resonance
; pollutant transport
; pollution effect
; pollution exposure
; recycling
; statistical analysis
; surface water
; wastewater treatment
; wastewater treatment plant
; water quality
; water resource
; animal cell
; Article
; controlled study
; effluent toxicity
; environmental exposure
; hydrophilicity
; laboratory test
; lipophilicity
; liver cell
; mass fragmentography
; metabolite
; metabolome
; metabolomics
; nonhuman
; priority journal
; proton nuclear magnetic resonance
; waste water management
; waste water recycling
; waste water treatment plant
; water pollutant
; water quality
; watershed
; zebra fish
; ZFL cell line
; Danio rerio
英文摘要: Cell-based metabolomics was used in a proof-of-concept fashion to investigate the biological effects of contaminants as they traveled from a wastewater treatment plant (WWTP) discharge to a drinking water treatment plant (DWTP) intake in a surface-water usage cycle. Zebrafish liver (ZFL) cells were exposed to water samples collected along a surface-water flowpath, where a WWTP was located ∼14.5 km upstream of a DWTP. The sampling sites included: 1) upstream of the WWTP, 2) the WWTP effluent discharging point, 3) a proximal location downstream of the WWTP outfall, 4) a distal location downstream of the WWTP outfall, 5) the drinking water intake, and 6) the treated drinking water collected prior to discharge to the distribution system. After a 48-h laboratory exposure, the hydrophilic and lipophilic metabolites in ZFL cell extracts were analyzed by proton nuclear magnetic resonance (1H NMR) spectroscopy and gas chromatography-mass spectrometry (GC-MS), respectively. Multivariate statistical analysis revealed distinct changes in metabolite profiles in response to WWTP effluent exposure. These effects on the hydrophilic metabolome gradually diminished downstream of the WWTP, becoming non-significant at the drinking water intake (comparable to upstream of the WWTP, p = 0.98). However, effects on the lipophilic metabolome increased significantly as the river flowed from the distal location downstream of the WWTP to the drinking water intake (p < 0.001), suggesting a source of bioactive compounds in this watershed other than the WWTP. ZFL cells exposed to treated drinking water did not exhibit significant changes in either the hydrophilic (p = 0.15) or lipophilic metabolome (p = 0.83) compared to the upstream site, suggesting that constituents in the WWTP effluent were efficiently removed by the drinking water treatment process. Impacts on ZFL cells from the WWTP effluent included disrupted energy metabolism, a global decrease in amino acids, and altered lipid metabolism pathways. Overall, this study demonstrated the utility of cell-based metabolomics as an effective tool for assessing the biological effects of complex pollutant mixtures, particularly when used as a complement to conventional chemical monitoring. © 2018 Citation statistics:
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/112438
Appears in Collections: 气候减缓与适应
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作者单位: U.S. Environmental Protection Agency, National Exposure Research Laboratory, Athens, GA 30605, United States; U.S. Environmental Protection Agency, National Exposure Research Laboratory, Cincinnati, OH 45268, United States; U.S. Environmental Protection Agency, National Risk Management Research Laboratory, Cincinnati, OH 45268, United States; U.S. Geological Survey, National Water Quality Laboratory, Denver Federal Center, Bldg 95, Denver, CO 80225, United States; U.S. Geological Survey, Central Midwest Water Science Center, 400 S. Clinton St, Rm 269 Federal Building, Iowa City, IA 52240, United States
Recommended Citation:
Zhen H.,Ekman D.R.,Collette T.W.,et al. Assessing the impact of wastewater treatment plant effluent on downstream drinking water-source quality using a zebrafish (Danio Rerio) liver cell-based metabolomics approach[J]. Water Research,2018-01-01,145