DOI: 10.1016/j.watres.2018.07.067
Scopus记录号: 2-s2.0-85051410609
论文题名: First insights into the living groundwater mycobiome of the terrestrial biogeosphere
作者: Nawaz A. ; Purahong W. ; Lehmann R. ; Herrmann M. ; Totsche K.U. ; Küsel K. ; Wubet T. ; Buscot F.
刊名: Water Research
ISSN: 431354
出版年: 2018
卷: 145 起始页码: 50
结束页码: 61
语种: 英语
英文关键词: Critical zone exploratory
; Fungal diversity
; Groundwater
; ITS
; Living fungi
; Subsurface biosphere
Scopus关键词: Aquatic ecosystems
; Aquatic organisms
; Aquifers
; Groundwater
; Groundwater resources
; Hydrogeology
; Risk assessment
; RNA
; Throughput
; Water quality
; Biogeochemical cycling
; Critical zones
; Environmental factors
; Fungal diversity
; Internal transcribed spacers
; Operational taxonomic units
; Subsurface biosphere
; Water quality monitoring
; Fungi
; ammonia
; ground water
; internal transcribed spacer
; nitrogen
; oxygen
; aquifer
; biosphere
; environmental factor
; fungus
; genetic marker
; groundwater
; molecular analysis
; pathogen
; research method
; species diversity
; taxonomy
; animal disease
; aquifer
; Article
; Ascomycetes
; Basidiomycetes
; biogeochemical cycling
; chemical analysis
; Chytridiomycetes
; environmental factor
; fungal community
; high throughput sequencing
; mycobiome
; nonhuman
; plant disease
; priority journal
; saprotroph
; taxonomy
; terrestrial surface waters
; water sampling
; Animalia
; Ascomycota
; Basidiomycota
; Chytridiomycota
; Fungi
; Otus
英文摘要: Although fungi play important roles in biogeochemical cycling in aquatic ecosystems and have received a great deal of attention, much remains unknown about the living fractions of fungal communities in aquifers of the terrestrial subsurface in terms of diversity, community dynamics, functional roles, the impact of environmental factors and presence of fungal pathogens. Here we address this gap in knowledge by using RNA-based high throughput pair-end illumina sequencing analysis of fungal internal transcribed spacer (ITS) gene markers, to target the living fractions of groundwater fungal communities from fractured alternating carbonate-/siliciclastic-rock aquifers of the Hainich Critical Zone Exploratory. The probed levels of the hillslope multi-storey aquifer system differ primarily in their oxygen and nitrogen content due to their different connections to the surface. We discovered highly diverse living fungal communities (384 Operational Taxonomic Units, OTUs) with different taxonomic affiliations and ecological functions. The observed fungal communities primarily belonged to three phyla: Ascomycota, Basidiomycota and Chytridiomycota. Perceived dynamics in the composition of living fungal communities were significantly shaped by the concentration of ammonium in the moderately agriculturally impacted aquifer system. Apart from fungal saprotrophs, we also detected living plant and animal pathogens for the first time in this aquifer system. This work also demonstrates that the RNA-based high throughput pair-end illumina sequencing method can be used in future for water quality monitoring in terms of living fungal load and subsequent risk assessments. In general, this study contributes towards the growing knowledge of aquatic fungi in terrestrial subsurface biogeosphere. © 2018 The Authors Citation statistics:
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/112457
Appears in Collections: 气候减缓与适应
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作者单位: Helmholtz Centre for Environmental Research – UFZ, Department of Soil Ecology, Halle (Saale), Germany; Helmholtz Centre for Environmental Research – UFZ, Department of Community Ecology, Halle (Saale), Germany; Department of Biology, University of Leipzig, Leipzig, Germany; Institute of Geosciences, Friedrich Schiller University Jena, Burgweg 11, Jena, 07749, Germany; Institute of Biodiversity, Friedrich Schiller University Jena, Dornburger Straße 159, Jena, 07743, Germany; German Centre for Integrative Biodiversity Research (iDiv) Halle-Jena-Leipzig, Deutscher Platz 5e, Leipzig, 04103, Germany
Recommended Citation:
Nawaz A.,Purahong W.,Lehmann R.,et al. First insights into the living groundwater mycobiome of the terrestrial biogeosphere[J]. Water Research,2018-01-01,145