DOI: 10.1016/j.watres.2018.07.059
Scopus记录号: 2-s2.0-85050894534
论文题名: Composition of heterotrophic specialized sub-guilds defined by a positive RNA and polyhydroxyalkanoate correlation in activated sludge
作者: Guo B. ; Manchester M. ; Luby T. ; Frigon D.
刊名: Water Research
ISSN: 431354
出版年: 2018
卷: 144 起始页码: 561
结束页码: 571
语种: 英语
英文关键词: Fluorescence-activated flow cytometry cell sorting
; High-throughput sequencing
; Microbial community structure
; Polyhydroxyalkanoate
; RNA levels
; Wastewater treatment modeling
Scopus关键词: Activated sludge process
; Flow cytometry
; Reclamation
; Structure (composition)
; Wastewater treatment
; Cell sorting
; High-throughput sequencing
; Microbial community structures
; Poly-hydroxyalkanoate
; Wastewater treatment models
; RNA
; bacterial DNA
; bacterial RNA
; polyhydroxyalkanoic acid
; RNA 16S
; activated sludge
; classification
; community structure
; flow cytometry
; fluorescence
; guild
; heterotrophy
; microbial community
; modeling
; molecular analysis
; organic compound
; phylogenetics
; RNA
; substrate
; wastewater treatment
; activated sludge
; Article
; Bacillus subtilis
; bacterial strain
; Caulobacterales
; cell selection
; community structure
; Cupriavidus necator
; Escherichia coli K 12
; Flavobacteriaceae
; flow cytometry
; heterotroph
; high throughput sequencing
; microbial community
; nonhuman
; phylogeny
; plant
; priority journal
; process optimization
; Pseudomonadaceae
; pyrosequencing
; Rhodobacterales
; Rhodococcus jostii
; RNA gene
; species composition
; waste water management
; Xanthomonadaceae
英文摘要: Microbial heterotrophic guilds in activated sludge wastewater treatment systems have complex population structures and functions. A previously proposed heterotrophic-specialist model states that heterotrophs consist of sub-guilds specialized in consuming specific classes of compounds, either readily degradable substrate (RDS) or slowly degradable substrate (SDS) according to current mathematical modeling practices for wastewater treatment processes. It follows from metabolic considerations that the levels of RNA and polyhydroxyalkanoate (PHA) are correlated for strains of the same species growing in different environments; a conjecture previously tested. The proposed classification of heterotrophs into RDS or SDS consumers predicts that the same correlation would also be found across heterotrophic species in conventional activated sludge systems; this prediction was tested in the current study. The positive correlation between the RNA and PHA levels was observed in 9 conventional activated sludge plants in two independent sampling times and it was also found stable over a 6-month regular sampling period at one of these plants. Together, these results imply that the levels of RNA and PHA can be used to define heterotrophic-specialist sub-guilds. In order to gain insight in the species composition of the defined sub-guilds, flow cytometry cell sorting was used to further analyze one of the activated sludge samples. Four sorted sub-samples were obtained (high-RNA/high-PHA, low-RNA/high-PHA, high-RNA/low-PHA, and low-RNA/low-PHA), and the phylogenetic composition of each was determined using 16S rRNA gene amplicon pyrosequencing. Heterotrophic genera were identified across 12 phyla, and their representation in each sorted sub-sample showed that the high-RNA/high-PHA and low-RNA/low-PHA groups were most dissimilar. The enriched genera in these sorted sub-samples are suggested to represent the composition of heterotrophic-specialized sub-guilds defined by the kinetics of substrate consumption. © 2018 Elsevier Ltd Citation statistics:
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/112522
Appears in Collections: 气候减缓与适应
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作者单位: Department of Civil Engineering and Applied Mechanics, McGill University, 817 Sherbrooke Street West, Montreal, Quebec H3A 0C3, Canada; Story Environmental and Geomatics, 332 Main St, Haileybury, Ontario P0J 1K0, Canada; Stantec, 200-325 25, Street SE, Calgary, Alberta T2A 7H8, Canada
Recommended Citation:
Guo B.,Manchester M.,Luby T.,et al. Composition of heterotrophic specialized sub-guilds defined by a positive RNA and polyhydroxyalkanoate correlation in activated sludge[J]. Water Research,2018-01-01,144