DOI: 10.1016/j.watres.2018.07.068
Scopus记录号: 2-s2.0-85053113150
论文题名: Tuning culturing conditions towards the production of neutral lipids from lubricant-based wastewater in open mixed bacterial communities
作者: Castro A.R. ; Silva P.T.S. ; Castro P.J.G. ; Alves E. ; Domingues M.R.M. ; Pereira M.A.
刊名: Water Research
ISSN: 431354
出版年: 2018
卷: 144 起始页码: 532
结束页码: 542
语种: 英语
英文关键词: Bacterial enrichment
; Bacterial lipid
; Cultivation conditions
; Oily wastewater
; Response surface methodology
; Triacylglycerol
Scopus关键词: Bacteria
; Biochemistry
; Biodegradation
; Biofuels
; Carbon
; Charge density waves
; Cultivation
; Food supply
; Glycerol
; Hydrocarbons
; Lipids
; Nitrogen
; Palmitic acid
; Regression analysis
; Saturated fatty acids
; Substrates
; Surface properties
; Waste management
; Wastewater treatment
; Bacterial lipids
; Cultivation conditions
; Oily wastewater
; Response surface methodology
; Triacylglycerols
; Linoleic acid
; bacterium
; chemical compound
; concentration (composition)
; cultivation
; feasibility study
; lipid
; lubricant
; microbial community
; response surface methodology
; wastewater treatment
; Acinetobacter
; Bacteria (microorganisms)
; Pseudomonas
; Rhodococcus
英文摘要: Production of bacterial lipid-based biofuels using inexpensive substrates, as wastes, is an emerging approach. In this work, a selective process using carbon feast-famine cycles was applied to obtain an indigenous microbial community of hydrocarbon-degrading and lipid-accumulating bacteria, using a real lubricant-based wastewater as carbon source. In the conditions applied, the enriched bacterial community, dominated by members of the genus Rhodococcus, Pseudomonas and Acinetobacter, was able to degrade almost all hydrocarbons present in the wastewater within 24 h’ incubation and to accumulate, although in low levels, triacylglycerol (TAG) (<5% of cell dry weight (CDW)) and polyhydroxyalkanoates (PHA) (3.8% ± 1.1% of the CDW) as well as an unknown lipid (29% ± 6% of CDW), presumably a wax ester-like compound. The influence of culture conditions, namely carbon and nitrogen concentrations (and C/N ratio) and cultivation time, on the amount and profile of produced storage compounds was further assessed using a statistical approach based on a central composite circumscribed design and surface response methodology. The regression analysis of the experimental design revealed that only nitrogen concentration and C/N ratio are significant for neutral lipid biosynthesis (p < 0.05). Maximum neutral lipid content, i.e. 33% (CDW basis), was achieved for the lowest carbon and nitrogen concentrations evaluated (10 g COD L−1 and 0.02 g N L−1). PHA accounted for less than 5% of CDW. In these conditions, neutral lipid content was mainly composed by TAG, about 70% (w/w). TAG precursors, namely monoacylglycerols (MAG), diacylglycerols (DAG) and fatty acids (FA), accounted for 22% of total neutral lipids and WE for about 7%. Nevertheless, according to the applied response surface model, further improvement of neutral lipids content is still possible if even lower nitrogen concentrations are used. The fatty acids detected in TAG extracts ranged from myristic acid (C14:0) to linoleic acid (C18:2), being the most abundant palmitic acid (C16:0), stearic acid (C18:0) and oleic acid (C18:1). This study shows the feasibility of combining treatment of hydrocarbon contaminated wastewater, herein demonstrated for lubricant-based wastewater, with the production of bacterial neutral lipids using open mixed bacterial communities. This approach can decrease the costs associated to both processes and contribute to a more sustainable waste management and production of lipid-based biofuels. © 2018 Elsevier Ltd Citation statistics:
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/112537
Appears in Collections: 气候减缓与适应
There are no files associated with this item.
作者单位: CEB - Centre of Biological Engineering, University of Minho, Campus de Gualtar, Braga, 4710-57, Portugal; Mass Spectrometry Centre, Department of Chemistry & QOPNA, University of Aveiro, Campus Universitário de Santiago, Aveiro, 3810-193, Portugal; Department of Chemical Engineering, Loughborough University, Epinal way, Loughborough, Le11 3EP, United Kingdom; Instituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa, Av. da República, Oeiras, 2780-157, Portugal
Recommended Citation:
Castro A.R.,Silva P.T.S.,Castro P.J.G.,et al. Tuning culturing conditions towards the production of neutral lipids from lubricant-based wastewater in open mixed bacterial communities[J]. Water Research,2018-01-01,144