DOI: 10.1016/j.watres.2018.05.057
Scopus记录号: 2-s2.0-85049337089
论文题名: Dichloromethane biodegradation in multi-contaminated groundwater: Insights from biomolecular and compound-specific isotope analyses
作者: Hermon L. ; Denonfoux J. ; Hellal J. ; Joulian C. ; Ferreira S. ; Vuilleumier S. ; Imfeld G.
刊名: Water Research
ISSN: 431354
出版年: 2018
卷: 142 起始页码: 217
结束页码: 226
语种: 英语
英文关键词: Biodegradation
; Biomarkers
; Bioremediation
; Contaminated aquifers
; CSIA
; Dichloromethane
; Multi-contamination
Scopus关键词: Aquifers
; Biodegradation
; Biomarkers
; Bioremediation
; Contamination
; Dichloromethane
; Genes
; Groundwater resources
; Hydrogeology
; Impurities
; Isotopes
; Strain
; Compound specific isotope analysis
; Contaminated aquifer
; Contaminated groundwater
; CSIA
; Denitrifying conditions
; Ex situ biodegradations
; High-throughput sequencing
; Terminal electron acceptor process
; Groundwater pollution
; bacterial RNA
; carbon
; chloride
; dichloromethane
; ground water
; RNA 16S
; aquifer pollution
; bacterium
; biodegradation
; biomarker
; bioremediation
; ethane
; groundwater pollution
; industrial waste
; isotopic analysis
; isotopic fractionation
; methane
; molecular analysis
; solvent
; Article
; bacterial gene
; bacterial strain
; bacterium culture
; bacterium isolation
; biodegradation
; controlled study
; dcmA gene
; dhlA gene
; fractionation
; France
; high throughput sequencing
; Hyphomicrobium
; isotope analysis
; microbial diversity
; microcosm
; nonhuman
; priority journal
; water contamination
; France
; Hyphomicrobium sp.
英文摘要: Dichloromethane (DCM) is a widespread and toxic industrial solvent which often co-occurs with chlorinated ethenes at polluted sites. Biodegradation of DCM occurs under both oxic and anoxic conditions in soils and aquifers. Here we investigated in situ and ex situ biodegradation of DCM in groundwater sampled from the industrial site of Themeroil (France), where DCM occurs as a major co-contaminant of chloroethenes. Carbon isotopic fractionation (εC) for DCM ranging from −46 to −22‰ were obtained under oxic or denitrifying conditions, in mineral medium or contaminated groundwater, and for laboratory cultures of Hyphomicrobium sp. strain GJ21 and two new DCM-degrading strains isolated from the contaminated groundwater. The extent of DCM biodegradation (B%) in the aquifer, as evaluated by compound-specific isotope analysis (δ13C), ranged from 1% to 85% applying DCM-specific εC derived from reference strains and those determined in this study. Laboratory groundwater microcosms under oxic conditions showed DCM biodegradation rates of up to 0.1 mM·day−1, with concomitant chloride release. Dehalogenase genes dcmA and dhlA involved in DCM biodegradation ranged from below 4 × 102 (boundary) to 1 × 107 (source zone) copies L−1 across the contamination plume. High-throughput sequencing on the 16S rrnA gene in groundwater samples showed that both contaminant level and terminal electron acceptor processes (TEAPs) influenced the distribution of genus-level taxa associated with DCM biodegradation. Taken together, our results demonstrate the potential of DCM biodegradation in multi-contaminated groundwater. This integrative approach may be applied to contaminated aquifers in the future, in order to identify microbial taxa and pathways associated with DCM biodegradation in relation to redox conditions and co-contamination levels. © 2018 Elsevier Ltd Citation statistics:
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/112605
Appears in Collections: 气候减缓与适应
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作者单位: Université de Strasbourg, CNRS, GMGM UMR 7156, Department of Microbiology, Genomics and the Environment, Strasbourg, France; BRGM, Geomicrobiology and Environmental Monitoring Unit, Orléans, France; Service Recherche, Développement et Innovation-Communautés Microbiennes, GenoScreen Lille, France; Université de Strasbourg, CNRS/EOST, LHyGeS UMR 7517, Laboratory of Hydrology and Geochemistry of Strasbourg, Strasbourg, France
Recommended Citation:
Hermon L.,Denonfoux J.,Hellal J.,et al. Dichloromethane biodegradation in multi-contaminated groundwater: Insights from biomolecular and compound-specific isotope analyses[J]. Water Research,2018-01-01,142