DOI: 10.1016/j.watres.2018.06.010
Scopus记录号: 2-s2.0-85049341205
论文题名: A uniform bacterial growth potential assay for different water types
作者: Farhat N. ; Hammes F. ; Prest E. ; Vrouwenvelder J.
刊名: Water Research
ISSN: 431354
出版年: 2018
卷: 142 起始页码: 227
结束页码: 235
语种: 英语
英文关键词: Adenosine tri-phosphate (ATP)
; Assimilable organic carbon (AOC)
; Flow cytometry (FCM)
; Seawater
Scopus关键词: Assays
; Bacteria
; Beverages
; Effluents
; Extraction
; Flow cytometry
; Organic carbon
; Seawater
; Water quality
; Assimilable organic carbon
; Biological stability
; Bottled mineral waters
; Carbon concentrations
; Chlorinated tap water
; Microbial communities
; Nutrient availability
; Wastewater effluents
; Growth kinetics
; acetic acid
; adenosine triphosphate
; carbon
; mineral water
; organic carbon
; river water
; sea water
; tap water
; water
; bacterium
; bioassay
; biomonitoring
; biotechnology
; concentration (composition)
; flow cytometry
; growth
; microbial community
; nutrient availability
; organic carbon
; sampling
; seawater
; water quality
; Article
; bacterial cell
; bacterial growth
; bacterial strain
; effluent
; flow cytometry
; microbial community
; nonhuman
; priority journal
; soil extract
; waste water
; Bacteria (microorganisms)
英文摘要: The bacterial growth potential is important to understand and manage bacterial regrowth-related water quality concerns. Bacterial growth potential depends on growth promoting/limiting compounds, therefore, nutrient availability is the key factor governing bacterial growth potential. Selecting proper tools for bacterial growth measurement is essential for routine implementation of the growth potential measurement. This study proposes a growth potential assay that is universal and can be used for different water types and soil extract without restrictions of pure culture or cultivability of the bacterial strain. The proposed assay measures the sample bacterial growth potential by using the indigenous community as inocula. Flow cytometry (FCM) and adenosine tri-phosphate (ATP) were used to evaluate the growth potential of six different microbial communities indigenous to the sample being analyzed, with increasing carbon concentrations. Bottled mineral water, non-chlorinated tap water, seawater, river water, wastewater effluent and a soil organic carbon extract were analyzed. Results showed that indigenous bacterial communities followed normal batch growth kinetics when grown on naturally present organic carbon. Indigenous bacterial growth could detect spiked organic carbon concentrations as low as 10 μg/L. The indigenous community in all samples responded proportionally to the increase in acetate-carbon and proportional growth could be measured with both FCM and ATP. Bacterial growth was proportional to the carbon concentration but not the same proportion factor for the different water samples tested. The effect of inoculating the same water with different indigenous microbial communities on the growth potential was also examined. The FCM results showed that the highest increase in total bacterial cell concentration was obtained with bacteria indigenous to the water sample. The growth potential assay using indigenous bacterial community revealed consistent results of bacterial growth in all the different samples tested and therefore providing a fast, more stable, and accurate approach for monitoring the biological stability of waters compared to the previously developed assays. The growth potential assay can be used to aid in detecting growth limitations by compounds other than organic carbon. © 2018 Elsevier Ltd Citation statistics:
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/112619
Appears in Collections: 气候减缓与适应
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作者单位: King Abdullah University of Science and Technology (KAUST), Water Desalination and Reuse Center (WDRC), Division of Biological and Environmental Science and Engineering (BESE), Thuwal, 23955-6900, Saudi Arabia; Eawag, Swiss Federal Institute of Aquatic Science and Technology, Dübendorf, CH-8600, Switzerland; Delft University of Technology, Faculty of Applied Sciences, Department of Biotechnology, Van der Maasweg 9, HZ Delft, 2629, Netherlands; Wetsus, European Centre of Excellence for Sustainable Water Technology, Oostergoweg 9, MA Leeuwarden, 8911, Netherlands
Recommended Citation:
Farhat N.,Hammes F.,Prest E.,et al. A uniform bacterial growth potential assay for different water types[J]. Water Research,2018-01-01,142