DOI: 10.1016/j.watres.2018.05.049
Scopus记录号: 2-s2.0-85050811057
论文题名: Simultaneous biogas upgrading and biochemicals production using anaerobic bacterial mixed cultures
作者: Omar B. ; Abou-Shanab R. ; El-Gammal M. ; Fotidis I.A. ; Kougias P.G. ; Zhang Y. ; Angelidaki I.
刊名: Water Research
ISSN: 431354
出版年: 2018
卷: 142 起始页码: 86
结束页码: 95
语种: 英语
英文关键词: 16S rRNA genes
; Acetogens
; Biogas upgrading
; Biomethane
; CO2 mitigation
; Gas fermentation
Scopus关键词: Bacteria
; Biogas
; Carbon dioxide
; Fuels
; Genes
; Methanogens
; Natural gas vehicles
; RNA
; 16S rRNA gene
; Acetogens
; Biogas upgrading
; Biomethane
; CO2 mitigation
; Gas fermentations
; Volatile fatty acids
; biogas
; genomic DNA
; RNA 16S
; volatile fatty acid
; abundance
; anoxic conditions
; biochemistry
; biogas
; carbon dioxide
; fermentation
; gene
; gene expression
; inoculation
; methane
; methanogenesis
; methanogenic bacterium
; microbial community
; natural gas
; anaerobic bacterium
; anaerobic fermentation
; Article
; bacterium culture
; chemical oxygen demand
; comparative study
; DNA extraction
; gene sequence
; inoculation
; methanogen
; methanogenesis
; methanogenic archaeon
; microbial community
; mixed cell culture
; nonhuman
; pH
; priority journal
; RNA analysis
; waste water treatment plant
; Bacteria (microorganisms)
英文摘要: A novel biological process to upgrade biogas was developed and optimised during the current study. In this process, CO2 in the biogas and externally provided H2 were fermented under mesophilic conditions to volatile fatty acids (VFAs), which are building blocks of higher-value biofuels. Meanwhile, the biogas was upgraded to biomethane (CH4 >95%), which can be used as a vehicle fuel or injected into the natural gas grid. To establish an efficient fermentative microbial platform, a thermal (at two different temperatures of 70 °C and 90 °C) and a chemical pretreatment method using 2-bromoethanesulfonate were investigated initially to inhibit methanogenesis and enrich the acetogenic bacterial inoculum. Subsequently, the effect of different H2:CO2 ratios on the efficiency of biogas upgrading and production of VFAs were further explored. The composition of the microbial community under different treatment methods and gas ratios has also been unravelled using 16S rRNA analysis. The chemical treatment of the inoculum had successfully blocked the activity of methanogens and enhanced the VFAs production, especially acetate. The chemical treatment led to a significantly better acetate production (291 mg HAc/L) compared to the thermal treatment. Based upon 16S rRNA gene sequencing, it was found that H2-utilizing methanogens were the dominant species in the thermally treated inoculum, while a significantly lower abundance of methanogens was observed in the chemically treated inoculum. The highest biogas content (96% (v/v)) and acetate production were achieved for 2H2:1CO2 ratio (v/v), with Acetoanaerobium noterae, as the dominant homoacetogenic hydrogen scavenger. Results from the present study can pave the way towards more development with respect to microorganisms and conditions for high efficient VFAs production and biogas upgrading. © 2018 Elsevier Ltd Citation statistics:
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/112625
Appears in Collections: 气候减缓与适应
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作者单位: Department of Environmental Engineering, Building 113, Technical University of Denmark, Lyngby, DK-2800, Denmark; Department of Environmental Sciences, Faculty of Science, Damietta University, Damietta, 34517, Egypt; Department of Environmental Biotechnology, City of Scientific Research and Technology Applications, Alexandria, 21934, Egypt
Recommended Citation:
Omar B.,Abou-Shanab R.,El-Gammal M.,et al. Simultaneous biogas upgrading and biochemicals production using anaerobic bacterial mixed cultures[J]. Water Research,2018-01-01,142