DOI: 10.1016/j.watres.2018.03.040
Scopus记录号: 2-s2.0-85049331293
论文题名: Transformation, CO2 formation and uptake of four organic micropollutants by carrier-attached microorganisms
作者: Falås P. ; Jewell K.S. ; Hermes N. ; Wick A. ; Ternes T.A. ; Joss A. ; Nielsen J.L.
刊名: Water Research
ISSN: 431354
出版年: 2018
卷: 141 起始页码: 405
结束页码: 416
语种: 英语
英文关键词: Biological wastewater treatment
; Microautoradiography
; Micropollutants
; Moving bed biofilm reactors
; Transformation
Scopus关键词: Biofilms
; Biological water treatment
; Carbon dioxide
; Fish
; Reclamation
; RNA
; Wastewater treatment
; Biological waste water treatment
; Microautoradiography
; Micropollutants
; Moving bed biofilm reactors
; Transformation
; Microorganisms
; carbon dioxide
; diclofenac
; ibuprofen
; mecoprop
; naproxen
; RNA 16S
; 2 (4 chloro 2 methylphenoxy)acetic acid
; carbon dioxide
; diclofenac
; ibuprofen
; mecoprop
; naproxen
; RNA 16S
; biofilm
; biological uptake
; biotechnology
; biotransformation
; carbon dioxide
; instrumentation
; microbial community
; microorganism
; municipal solid waste
; organic pollutant
; radiography
; wastewater treatment plant
; amplicon
; Article
; autoradiography
; biofilm
; biotransformation
; controlled study
; Deltaproteobacteria
; Firmicutes
; fluorescence in situ hybridization
; Gammaproteobacteria
; gene sequence
; liquid chromatography-mass spectrometry
; liquid scintillation counting
; microbial community
; nonhuman
; pollutant
; priority journal
; waste water management
; analogs and derivatives
; Deltaproteobacteria
; Firmicutes
; Gammaproteobacteria
; genetics
; metabolism
; microbiology
; physiology
; procedures
; sewage
; waste water
; water pollutant
; Deltaproteobacteria
; Firmicutes
; Gammaproteobacteria
; 2-Methyl-4-chlorophenoxyacetic Acid
; Biofilms
; Carbon Dioxide
; Deltaproteobacteria
; Diclofenac
; Firmicutes
; Gammaproteobacteria
; Ibuprofen
; In Situ Hybridization, Fluorescence
; Naproxen
; RNA, Ribosomal, 16S
; Waste Disposal, Fluid
; Waste Water
; Water Microbiology
; Water Pollutants, Chemical
英文摘要: A tiered process was developed to assess the transformation, CO2 formation and uptake of four organic micropollutants by carrier-attached microorganisms from two municipal wastewater treatment plants. At the first tier, primary transformation of ibuprofen, naproxen, diclofenac, and mecoprop by carrier-attached microorganisms was shown by the dissipation of the target compounds and the formation of five transformation products using LC-tandem MS. At the second tier, the microbial cleavage of the four organic micropollutants was confirmed with 14C-labeled micropollutants through liquid scintillation counting of the 14CO2 formed. At the third tier, microautoradiography coupled with fluorescence in situ hybridization (MAR-FISH) was used to screen carrier-attached microorganisms for uptake of the four radiolabeled micropollutants. Results from the MAR-FISH screening indicated that only a small fraction of the microbial community (≤1‰) was involved in the uptake of the radiolabeled micropollutants and that the responsible microorganisms differed between the compounds. At the fourth tier, the microbial community structure of the carrier-attached biofilms was analyzed by 16S rRNA gene amplicon sequencing. The sequencing results showed that the MAR-FISH screening targeted ∼80% of the microbial community and that several taxonomic families within the FISH-probed populations with MAR-positive signals (i.e. Firmicutes, Gammaproteobacteria, and Deltaproteobacteria) were present in both biofilms. From the broader perspective of organic micropollutant removal in biological wastewater treatment, the MAR-FISH results of this study indicate a high degree of microbial substrate specialization that could explain differences in transformation rates and patterns between micropollutants and microbial communities. © 2018 Elsevier Ltd Citation statistics:
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/112671
Appears in Collections: 气候减缓与适应
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作者单位: Eawag, Swiss Federal Institute of Aquatic Science and Technology, Überlandstrasse 133, Dübendorf, 8600, Switzerland; Water and Environmental Engineering, Department of Chemical Engineering, Lund University, Lund, 221 00, Sweden; Federal Institute of Hydrology, Am Mainzer Tor 1, Koblenz, 56068, Germany; Center for Microbial Communities, Department of Chemistry and Bioscience, Aalborg University, Fredrik Bajers Vej 7H, Aalborg, 9220, Denmark
Recommended Citation:
Falås P.,Jewell K.S.,Hermes N.,et al. Transformation, CO2 formation and uptake of four organic micropollutants by carrier-attached microorganisms[J]. Water Research,2018-01-01,141