DOI: 10.1016/j.watres.2018.04.027
Scopus记录号: 2-s2.0-85045885766
论文题名: Differential development of Legionella sub-populations during short- and long-term starvation
作者: Schrammel B. ; Cervero-Aragó S. ; Dietersdorfer E. ; Walochnik J. ; Lück C. ; Sommer R. ; Kirschner A.
刊名: Water Research
ISSN: 431354
出版年: 2018
卷: 141 起始页码: 417
结束页码: 427
语种: 英语
英文关键词: Esterase activity
; Flow cytometry
; Legionella
; Membrane integrity
; Starvation
; Viable but nonculturable
Scopus关键词: Cells
; Enzymes
; Esters
; Flow cytometry
; Strain
; Water analysis
; Waterworks
; Esterase activities
; Legionella
; Membrane integrity
; Starvation
; Viable but non-culturable
; Cytology
; esterase
; leucine
; esterase
; bacterium
; bioindicator
; cell
; concentration (composition)
; detection method
; engineering
; enzyme activity
; flow cytometry
; membrane
; microbial community
; monitoring system
; starvation
; subpopulation
; Article
; cell count
; cell membrane
; cell population
; controlled study
; enzyme activity
; Legionella
; limit of detection
; limit of quantitation
; microcosm
; nonhuman
; priority journal
; starvation
; growth, development and aging
; metabolism
; microbial viability
; microbiological examination
; Legionella
; Legionella pneumophila
; Bacteriological Techniques
; Esterases
; Legionella
; Microbial Viability
英文摘要: Legionellae are among the most important waterborne pathogens in industrialized countries. Monitoring and surveillance of Legionella in engineered water systems is usually performed with culture-based methods. Since the advent of culture-independent techniques, it has become clear that Legionella concentrations are often several orders of magnitude higher than those measured by culture-based techniques and that a variable proportion of these non-culturable cells are viable. In engineered water systems, the formation of these viable but non-culturable (VBNC) cells can be caused by different kinds of stress, such as, and most importantly, nutrient starvation, oxidative stress and heat. In this study, the formation of VBNC cells of six Legionella strains under conditions of starvation was monitored in mono-species microcosms for up to one year using a combination of different viability indicators. Depending on the strain, complete loss of culturability was observed from 11 days to 8 weeks. During the starvation process, three distinct phases and different sub-populations of VBNC cells were identified. Until complete loss of culturability, the number of membrane-intact cells decreased rapidly to 5.5–69% of the initial cell concentration. The concentration of the sub-population with low esterase activity dropped to 0.03–55%, and the concentration of the highly esterase-active sub-population dropped to 0.01–1.2% of the initial concentration; these sub-populations remained stable for several weeks to months. Only after approximately 200 days of starvation, the number of VBNC cells started to decrease below detection limits. The most abundant VBNC sub-populations were characterized by partially damaged membranes and low esterase-activity. With this study, we showed that upon starvation, a stable VBNC Legionella community may be present over several months in a strain-dependent manner even under harsh conditions. Even after one year of starvation, a small proportion of L. pneumophila cells with high esterase-activity was detected. We speculate that this highly active VBNC subpopulation is able to infect amoebae and human macrophages. © 2018 The Authors Citation statistics:
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/112680
Appears in Collections: 气候减缓与适应
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作者单位: Institute for Hygiene and Applied Immunology, Water Hygiene, Center for Pathophysiology, Infectiology and Immunology, Medical University of Vienna, Austria; Institute for Specific Prophylaxis and Tropical Medicine, Center for Pathophysiology, Infectiology and Immunology, Medical University of Vienna, Austria; Institute for Medical Microbiology and Hygiene, Medical Faculty “Carl Gustav Carus”, Technical University Dresden, Germany; Interuniversity Cooperation Centre for Water and Health, Austria
Recommended Citation:
Schrammel B.,Cervero-Aragó S.,Dietersdorfer E.,et al. Differential development of Legionella sub-populations during short- and long-term starvation[J]. Water Research,2018-01-01,141