DOI: 10.1016/j.watres.2018.05.004
Scopus记录号: 2-s2.0-85047099182
论文题名: Preferential feeding in Naegleria fowleri; intracellular bacteria isolated from amoebae in operational drinking water distribution systems
作者: Miller H.C. ; Morgan M.J. ; Walsh T. ; Wylie J.T. ; Kaksonen A.H. ; Puzon G.J.
刊名: Water Research
ISSN: 431354
出版年: 2018
卷: 141 起始页码: 126
结束页码: 134
语种: 英语
英文关键词: Amoeba
; Ecogenomics
; Intracellular bacteria
; Meiothermus
; Naegleria
; Naegleria fowleri
Scopus关键词: Bacteria
; Biofilms
; Ecology
; Protozoa
; RNA
; Water distribution systems
; Amoeba
; Ecogenomics
; Intracellular bacteria
; Meiothermus
; Naegleria
; Naegleria fowleri
; Potable water
; drinking water
; RNA 16S
; bacterium
; biofilm
; colonization
; distribution system
; drinking water
; genetic analysis
; genomics
; microbial ecology
; mortality
; operations technology
; protist
; public health
; amoeba (life cycle stage)
; amplicon
; Article
; bacterial colonization
; bacterium
; bacterium contamination
; bacterium isolation
; biofilm
; biomass
; chemical parameters
; ecosystem monitoring
; fluorescence microscopy
; Meiothermus chliarophilus
; microbial community
; Naegleria fowleri
; nonhuman
; polymerase chain reaction
; priority journal
; sample
; Sanger sequencing
; water supply
; Deinococcus
; environmental monitoring
; genetics
; isolation and purification
; microbiology
; Naegleria fowleri
; physiology
; water pollutant
; United States
; Amoeba (genus)
; Meiothermus
; Meiothermus chliarophilus
; Meiothermus ruber
; Naegleria
; Naegleria fowleri
; Biofilms
; Deinococcus
; Drinking Water
; Environmental Monitoring
; Naegleria fowleri
; RNA, Ribosomal, 16S
; Water Pollutants
英文摘要: The amoeba Naegleria fowleri is the causative agent of the highly fatal disease, primary amoebic meningoencephalitis, and estimated to cause 16 deaths per year in the United States alone. Colonisation of drinking water distribution systems (DWDSs) by the N. fowleri is a significant public health issue. Understanding the factors which enable this pathogen to colonise and thrive in DWDSs is critical for proper management. The microbial ecology within DWDSs may influence the ability of N. fowleri to colonise DWDSs by facilitating the availability of an appropriate food source. Using biofilm samples obtained from operational DWDSs, 16S rRNA amplicon metabarcoding was combined with genus-specific PCR and Sanger sequencing of intracellular associated bacteria from isolated amoeba and their parental biofilms to identify Meiothermus chliarophilus as a potential food source for N. fowleri. Meiothermus was confirmed as a food source for N. fowleri following successful serial culturing of axenic N. fowleri with M. chliarophilus or M. ruber as the sole food source. The ability to identify environmental and ecological conditions favourable to N. fowleri colonisation, including the detection of appropriate food sources such as Meiothermus, could provide water utilities with a predictive tool for managing N. fowleri colonisation within the DWDS. © 2018 Elsevier Ltd Citation statistics:
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/112686
Appears in Collections: 气候减缓与适应
There are no files associated with this item.
作者单位: CSIRO Land and Water, Private Bag No.5, Wembley, Western Australia 6913, Australia; School of Biomedical Sciences, University of Western Australia, 35 Stirling Highway, Crawley, Western Australia 6009, Australia; CSIRO Land and Water, Black Mountain Laboratories, P.O. Box 1700, Canberra, ACT 2601, Australia
Recommended Citation:
Miller H.C.,Morgan M.J.,Walsh T.,et al. Preferential feeding in Naegleria fowleri; intracellular bacteria isolated from amoebae in operational drinking water distribution systems[J]. Water Research,2018-01-01,141