DOI: 10.1016/j.watres.2018.04.009
Scopus记录号: 2-s2.0-85047404859
论文题名: In vitro bioanalysis of drinking water from source to tap
作者: Rosenmai A.K. ; Lundqvist J. ; le Godec T. ; Ohlsson Å. ; Tröger R. ; Hellman B. ; Oskarsson A.
刊名: Water Research
ISSN: 431354
出版年: 2018
卷: 139 起始页码: 272
结束页码: 280
语种: 英语
英文关键词: Aryl hydrocarbon receptor
; Bioassay
; Drinking water treatment
; Effect-based assessment
; Estrogen receptor
; Nrf2
Scopus关键词: Activated carbon
; Activated carbon treatment
; Bioactivity
; Bioassay
; Chlorine compounds
; Hydrocarbons
; Phase separation
; Potable water
; Surface waters
; Water pollution
; Water quality
; Aryl hydrocarbon receptor
; Effect-based assessment
; Estrogen receptor
; Granulated activated carbons
; Nrf2
; Nuclear factor erythroid 2-related factor 2
; Peroxisome proliferator-activated receptor
; Solid-phase extraction
; Chemical water treatment
; activated carbon
; androgen receptor
; aromatic hydrocarbon receptor
; drinking water
; estrogen receptor
; peroxisome proliferator activated receptor alpha
; tap water
; transcription factor Nrf2
; drinking water
; NFE2L2 protein, human
; activated carbon
; bioactivity
; bioassay
; biological method
; chemical pollutant
; disinfection
; drinking water
; estrogenic compound
; extraction method
; hydrocarbon
; laboratory method
; oxidative stress
; pollutant removal
; water quality
; Article
; bioassay
; biological activity
; comet assay
; disinfection
; DNA damage
; in vitro study
; oxidative stress
; priority journal
; solid phase extraction
; water analysis
; water contamination
; water quality
; water sampling
; water treatment
; analysis
; animal
; CHO cell line
; Cricetulus
; filtration
; genetics
; human
; tumor cell line
; water management
; water pollutant
; Animals
; Cell Line, Tumor
; CHO Cells
; Comet Assay
; Cricetulus
; Disinfection
; Drinking Water
; Filtration
; Humans
; NF-E2-Related Factor 2
; PPAR alpha
; Receptors, Androgen
; Receptors, Aryl Hydrocarbon
; Receptors, Estrogen
; Water Pollutants, Chemical
; Water Purification
英文摘要: The presence of chemical pollutants in sources of drinking water is a key environmental problem threatening public health. Efficient removal of pollutants in drinking water treatment plants (DWTPs) is needed as well as methods for assessment of the total impact of all present chemicals on water quality. In the present study we have analyzed the bioactivity of water samples from source to tap, including effects of various water treatments in a DWTP, using a battery of cell-based bioassays, covering health-relevant endpoints. Reporter gene assays were used to analyze receptor activity of the aryl hydrocarbon receptor (AhR), estrogen receptor (ER), androgen receptor (AR), peroxisome proliferator-activated receptor alpha (PPARα) and induction of oxidative stress by the nuclear factor erythroid 2-related factor 2 (Nrf2). DNA damage was determined by Comet assay. Grab water samples were concentrated by HLB or ENV solid phase extraction and the water samples assayed at a relative enrichment factor of 50. The enrichment procedure did not induce any bioactivity. No bioactivity was detected in Milli-Q water or drinking water control samples. Induction of AhR, ER and Nrf2 activities was revealed in source to tap water samples. No cytotoxicity, PPARα or AR antagonist activity, or DNA damage were observed in any of the water samples. A low AR agonist activity was detected in a few samples of surface water, but not in the samples from the DWTP. The treatment steps at the DWTP, coagulation, granulated activated carbon filtration, UV disinfection and NH2Cl dosing had little or no effect on the AhR, Nrf2 and ER bioactivity. However, nanofiltration and passage through the distribution network drastically decreased AhR activity, while the effect on Nrf2 activity was more modest and no apparent effect was observed on ER activity. The present results suggest that bioassays are useful tools for evaluation of the efficiency of different treatment steps in DWTPs in reducing toxic activities. Bioassays of AhR and Nrf2 are useful for screening of effects of a broad range of chemicals in drinking water and ER activity can be monitored with a high sensitivity. © 2018 Elsevier Ltd Citation statistics:
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/112730
Appears in Collections: 气候减缓与适应
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作者单位: Department of Biomedical Sciences and Veterinary Public Health, Swedish University of Agricultural Sciences, Box 7028, Uppsala, SE-750 07, Sweden; Department of Aquatic Sciences and Assessment, Swedish University of Agricultural Sciences, Box 7050, Uppsala, SE-750 07, Sweden; Department of Pharmaceutical Biosciences, Division of Drug Safety and Toxicology, Uppsala University, Box 591, Uppsala, 751 24, Sweden
Recommended Citation:
Rosenmai A.K.,Lundqvist J.,le Godec T.,et al. In vitro bioanalysis of drinking water from source to tap[J]. Water Research,2018-01-01,139