DOI: 10.1016/j.watres.2018.03.014
Scopus记录号: 2-s2.0-85044985950
论文题名: Reactor staging influences microbial community composition and diversity of denitrifying MBBRs- Implications on pharmaceutical removal
作者: Torresi E. ; Gülay A. ; Polesel F. ; Jensen M.M. ; Christensson M. ; Smets B.F. ; Plósz B.G.
刊名: Water Research
ISSN: 431354
出版年: 2018
卷: 138 起始页码: 333
结束页码: 345
语种: 英语
英文关键词: Heterotrophic denitrification
; Micropollutant removal
; Moving bed biofilm reactors
; Organic carbon
; Structure-function relationships
Scopus关键词: Biodiversity
; Biofilms
; Bioreactors
; Microorganisms
; Optimization
; Organic carbon
; Polymerase chain reaction
; Rate constants
; RNA
; Biotransformation kinetics
; Heterotrophic denitrification
; Microbial community composition
; Microbial community structures
; Micropollutant removals
; Moving bed biofilm reactors
; Single-stage configuration
; Structure-function relationship
; Denitrification
; carbon
; RNA 16S
; drug
; abundance
; bacterium
; biofilm
; bioreactor
; biotransformation
; community structure
; denitrification
; drug
; metabolism
; microbial activity
; microbial community
; microbial ecology
; organic carbon
; pollutant removal
; species diversity
; species richness
; amplicon
; Article
; biodiversity
; biotransformation
; Burkholderiales
; controlled study
; Flavobacteriales
; microbial community
; microbial diversity
; nonhuman
; polymerase chain reaction
; priority journal
; Sphingobacteriales
; structure activity relation
; Xanthomonadales
; bacterium
; biofilm
; bioreactor
; classification
; denitrification
; genetics
; metabolism
; microbiology
; sewage
; water pollutant
; Burkholderiales
; Deinococcales
; Flavobacteriales
; Otus
; Sphingobacteriales
; Xanthomonadales
; Bacteria
; Biodiversity
; Biofilms
; Bioreactors
; Carbon
; Denitrification
; Pharmaceutical Preparations
; RNA, Ribosomal, 16S
; Waste Disposal, Fluid
; Water Pollutants, Chemical
英文摘要: The subdivision of biofilm reactor in two or more stages (i.e., reactor staging) represents an option for process optimisation of biological treatment. In our previous work, we showed that the gradient of influent organic substrate availability (induced by the staging) can influence the microbial activity (i.e., denitrification and pharmaceutical biotransformation kinetics) of a denitrifying three-stage Moving Bed Biofilm Reactor (MBBR) system. However, it is unclear whether staging and thus the long-term exposure to varying organic carbon type and loading influences the microbial community structure and diversity. In this study, we investigated biofilm structure and diversity in the three-stage MBBR system (S) compared to a single-stage configuration (U) and their relationship with microbial functions. Results from 16S rRNA amplicon libraries revealed a significantly higher microbial richness in the staged MBBR (at 99% sequence similarity) compared to single-stage MBBR. A more even and diverse microbial community was selected in the last stage of S (S3), likely due to exposure to carbon limitation during continuous-flow operation. A core of OTUs was shared in both systems, consisting of Burkholderiales, Xanthomonadales, Flavobacteriales and Sphingobacteriales, while MBBR staging selected for specific taxa (i.e., Candidate division WS6 and Deinococcales). Results from quantitative PCR (qPCR) showed that S3 exhibited the lowest abundance of 16S rRNA but the highest abundance of atypical nosZ, suggesting a selection of microbes with more diverse N-metabolism (i.e., incomplete denitrifiers) in the stage exposed to the lowest carbon availability. A positive correlation (p < 0.05) was observed between removal rate constants of several pharmaceuticals with abundance of relevant denitrifying genes, but not with biodiversity. Despite the previously suggested positive relationship between microbial diversity and functionality in macrobial and microbial ecosystems, this was not observed in the current study, indicating a need to further investigate structure-function relationships for denitrifying systems. © 2018 Citation statistics:
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/112771
Appears in Collections: 气候减缓与适应
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作者单位: DTU Environment, Technical University of Denmark, Bygningstorvet B115, Kongens Lyngby, 2800, Denmark; Veolia Water Technologies AB, AnoxKaldnes, Klosterängsvägen 11A, Lund, SE-226 47, Sweden; Department of Chemical Engineering, University of Bath, Claverton Down, Bath, BA2 7AY, United Kingdom
Recommended Citation:
Torresi E.,Gülay A.,Polesel F.,et al. Reactor staging influences microbial community composition and diversity of denitrifying MBBRs- Implications on pharmaceutical removal[J]. Water Research,2018-01-01,138