DOI: 10.1016/j.watres.2018.03.032
Scopus记录号: 2-s2.0-85046015987
论文题名: Monitoring biofilm function in new and matured full-scale slow sand filters using flow cytometric histogram image comparison (CHIC)
作者: Chan S. ; Pullerits K. ; Riechelmann J. ; Persson K.M. ; Rådström P. ; Paul C.J.
刊名: Water Research
ISSN: 431354
出版年: 2018
卷: 138 起始页码: 27
结束页码: 36
语种: 英语
英文关键词: Biofilm
; Cytometric histogram image comparison (CHIC)
; Drinking water
; Flow cytometry
; Schmutzdecke
; Slow sand filters
Scopus关键词: Bandpass filters
; Biofilms
; Effluents
; Escherichia coli
; Flow cytometry
; Graphic methods
; Organic carbon
; Potable water
; Bacterial community
; Image comparison
; Microbial communities
; Microbial indicators
; Sand composition
; Schmutzdecke
; Slow sand filters
; Total Organic Carbon
; Sand
; drinking water
; silicon dioxide
; bacterium
; biofilm
; bioindicator
; comparative study
; drinking water
; filter
; flow cytometry
; image analysis
; microbial community
; microbial ecology
; Article
; bacterial cell
; bacterial count
; biofilm
; coliform bacterium
; controlled study
; cytometric histogram image comparison
; effluent
; Escherichia coli
; flow cytometry
; heterotroph
; image analysis
; microbial community
; next generation sequencing
; nonhuman
; pH
; priority journal
; sand
; total organic carbon
; water quality
; bacterial load
; bacterial phenomena and functions
; bacterium
; chemistry
; devices
; filtration
; flow cytometry
; isolation and purification
; procedures
; water management
; Bacteria (microorganisms)
; Escherichia coli
; Bacteria
; Bacterial Load
; Bacterial Physiological Phenomena
; Biofilms
; Filtration
; Flow Cytometry
; Silicon Dioxide
; Water Purification
英文摘要: While slow sand filters (SSFs) have produced drinking water for more than a hundred years, understanding of their associated microbial communities is limited. In this study, bacteria in influent and effluent water from full-scale SSFs were explored using flow cytometry (FCM) with cytometric histogram image comparison (CHIC) analysis; and routine microbial counts for heterotrophs, total coliforms and Escherichia coli. To assess if FCM can monitor biofilm function, SSFs differing in age and sand composition were compared. FCM profiles from two established filters were indistinguishable. To examine biofilm in the deep sand bed, SSFs were monitored during a scraping event, when the top layer of sand and the schmutzdecke are removed to restore flow through the filter. The performance of an established SSF was stable: total organic carbon (TOC), pH, numbers of heterotrophs, coliforms, E. coli, and FCM bacterial profile were unaffected by scraping. However, the performance of two newly-built SSFs containing new and mixed sand was compromised: breakthrough of both microbial indicators and TOC occurred following scraping. The compromised performance of the new SSFs was reflected in distinct effluent bacterial communities; and, the presence of microbial indicators correlated to influent bacterial communities. This demonstrated that FCM can monitor SSF performance. Removal of the top layer of sand did not alter the effluent water from the established SSF, but did affect that of the SSFs containing new sand. This suggests that the impact of the surface biofilm on effluent water is greater when the deep sand bed biofilm is not established. © 2018 The Authors Citation statistics:
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/112778
Appears in Collections: 气候减缓与适应
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作者单位: Applied Microbiology, Department of Chemistry, Lund University, P.O. Box 124, Lund, SE-221 00, Sweden; Sweden Water Research AB, Ideon Science Park, Scheelevägen 15, Lund, SE-223 70, Sweden; Sydvatten AB, Hyllie Stationstorg 21, Malmö, SE-215 32, Sweden; Water Resources Engineering, Department of Building and Environmental Technology, Lund University, P.O. Box 118, Lund, SE-221 00, Sweden
Recommended Citation:
Chan S.,Pullerits K.,Riechelmann J.,et al. Monitoring biofilm function in new and matured full-scale slow sand filters using flow cytometric histogram image comparison (CHIC)[J]. Water Research,2018-01-01,138