DOI: 10.1016/j.watres.2018.03.052
Scopus记录号: 2-s2.0-85044515122
论文题名: Rapid detection and quantification of the potentially toxic cyanobacterium Planktothrix rubescens by in-vivo fluorometry and flow cytometry
作者: Weisse T. ; Bergkemper V.
刊名: Water Research
ISSN: 431354
出版年: 2018
卷: 138 起始页码: 234
结束页码: 240
语种: 英语
英文关键词: Acoustic flow cytometry
; Cyanobacteria
; Harmful algal blooms
; In-vivo fluorometry
; Lakes
; Planktothrix rubescens
Scopus关键词: Environmental regulations
; Fluorometers
; Lakes
; Metallography
; Toxic materials
; Water conservation
; Cyanobacteria
; Cytometry
; Harmful algal blooms
; In-vivo
; Planktothrix rubescens
; Flow cytometry
; algal bloom
; concentration (composition)
; cyanobacterium
; detection method
; flow cytometry
; laboratory method
; lake water
; quantitative analysis
; sensor
; toxicity
; Article
; autumn
; bacterium detection
; cell density
; comparative study
; cyanobacterium
; environmental temperature
; flow cytometry
; fluorometry
; in vivo study
; light intensity
; nonhuman
; pH
; phytoplankton
; Planktothrix rubescens
; priority journal
; single cell analysis
; summer
; surface property
; Austria
; biomass
; cyanobacterium
; environmental monitoring
; flow cytometry
; fluorometry
; isolation and purification
; lake
; microbiology
; procedures
; water pollutant
; Austria
; Mondsee [Salzburg]
; Salzburg [Austria]
; algae
; Cyanobacteria
; Planktothrix rubescens
; Austria
; Biomass
; Cyanobacteria
; Environmental Monitoring
; Flow Cytometry
; Fluorometry
; Lakes
; Water Pollutants
英文摘要: We combined profiling of the bloom-forming and potentially toxigenic cyanobacterium Planktothrix rubescens using a multiparameter probe equipped with a phycoerythrin sensor (in vivo fluorometry, IVL) in Lake Mondsee, Austria, with flow cytometric live analyses of discrete samples taken from several depths in the upper 20 m of the water column. Results obtained by IVL and acoustic flow cytometry (AFC) were compared to microscopic analyses of integrated (0–21 m) water samples using fixed material. This comparison was made because the integrated samples are used for the Austrian monitoring programme according to the EU Water Framework Directive. We demonstrate that AFC provides quantitative analyses of the filaments of P. rubescens that are significantly correlated to IVF and microscopic analyses, allowing rapid (within hours) and more precise calculation of P. rubescens biomass than estimates derived from IVL. Our analysis shows that vertically integrated water samples provide unreliable information on the concentration of P. rubescens in the upper surface waters and on the peak concentration of P. rubescens within the water column. We conclude that the protocol that we developed is superior to the current monitoring practice. © 2018 Elsevier Ltd Citation statistics:
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/112779
Appears in Collections: 气候减缓与适应
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作者单位: Research Department for Limnology, University of Innsbruck, Mondseestraße 9, Mondsee, 5310, Austria
Recommended Citation:
Weisse T.,Bergkemper V.. Rapid detection and quantification of the potentially toxic cyanobacterium Planktothrix rubescens by in-vivo fluorometry and flow cytometry[J]. Water Research,2018-01-01,138