globalchange  > 气候减缓与适应
DOI: 10.1016/j.watres.2018.03.003
Scopus记录号: 2-s2.0-85044585777
论文题名:
Implementation options for DNA-based identification into ecological status assessment under the European Water Framework Directive
作者: Hering D.; Borja A.; Jones J.I.; Pont D.; Boets P.; Bouchez A.; Bruce K.; Drakare S.; Hänfling B.; Kahlert M.; Leese F.; Meissner K.; Mergen P.; Reyjol Y.; Segurado P.; Vogler A.; Kelly M.
刊名: Water Research
ISSN: 431354
出版年: 2018
卷: 138
起始页码: 192
结束页码: 205
语种: 英语
英文关键词: Biological quality elements ; eDNA ; Lakes ; Meta-barcoding ; Rivers ; Transitional and coastal waters
Scopus关键词: Animals ; Bar codes ; Cost effectiveness ; Environmental impact ; Environmental regulations ; Fish ; Fisheries ; Lakes ; Libraries ; Phytoplankton ; Rivers ; Water conservation ; Benthic invertebrates ; Coastal waters ; Conventional approach ; eDNA ; European Water Framework Directive ; Meta-barcoding ; Morphological identifications ; Taxonomic diversity ; DNA ; DNA ; lake water ; river water ; water ; DNA ; sea water ; assessment method ; coastal water ; DNA ; environmental impact ; identification method ; implementation process ; lake water ; morphology ; river water ; sampling ; water planning ; water quality ; angiosperm ; benthos ; coastal waters ; DNA barcoding ; DNA library ; fish ; gill ; invertebrate ; macroalga ; nonhuman ; phytoplankton ; priority journal ; Review ; water management ; animal ; DNA barcoding ; ecosystem ; environmental monitoring ; lake ; procedures ; river ; Invertebrata ; Animals ; DNA ; DNA Barcoding, Taxonomic ; Ecosystem ; Environmental Monitoring ; Fishes ; Invertebrates ; Lakes ; Phytoplankton ; Rivers ; Seawater
英文摘要: Assessment of ecological status for the European Water Framework Directive (WFD) is based on “Biological Quality Elements” (BQEs), namely phytoplankton, benthic flora, benthic invertebrates and fish. Morphological identification of these organisms is a time-consuming and expensive procedure. Here, we assess the options for complementing and, perhaps, replacing morphological identification with procedures using eDNA, metabarcoding or similar approaches. We rate the applicability of DNA-based identification for the individual BQEs and water categories (rivers, lakes, transitional and coastal waters) against eleven criteria, summarised under the headlines representativeness (for example suitability of current sampling methods for DNA-based identification, errors from DNA-based species detection), sensitivity (for example capability to detect sensitive taxa, unassigned reads), precision of DNA-based identification (knowledge about uncertainty), comparability with conventional approaches (for example sensitivity of metrics to differences in DNA-based identification), cost effectiveness and environmental impact. Overall, suitability of DNA-based identification is particularly high for fish, as eDNA is a well-suited sampling approach which can replace expensive and potentially harmful methods such as gill-netting, trawling or electrofishing. Furthermore, there are attempts to replace absolute by relative abundance in metric calculations. For invertebrates and phytobenthos, the main challenges include the modification of indices and completing barcode libraries. For phytoplankton, the barcode libraries are even more problematic, due to the high taxonomic diversity in plankton samples. If current assessment concepts are kept, DNA-based identification is least appropriate for macrophytes (rivers, lakes) and angiosperms/macroalgae (transitional and coastal waters), which are surveyed rather than sampled. We discuss general implications of implementing DNA-based identification into standard ecological assessment, in particular considering any adaptations to the WFD that may be required to facilitate the transition to molecular data. © 2018 Elsevier Ltd
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资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/112783
Appears in Collections:气候减缓与适应

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作者单位: University of Duisburg-Essen, Aquatic Ecology, Essen, 45117, Germany; AZTI, Marine Research Division, Pasaia, 20110, Spain; School of Biological and Chemical Sciences, Queen Mary University of London, London, E1 4NS, United Kingdom; Association VigiLIFE, France and Institute of Hydrobiology and Aquatic Ecosystem Management, University of Natural Resources and Life Sciences, 17, rue du Lac Saint-André Savoie Technolac - BP 274, 73375 Le Bourget-du-Lac Cedex, Gregor-Mendel-Strasse 33, Vienna, 1180, Austria; PCM, Provincial Centre of Environmental Research, Godshuizenlaan 95, Ghent, 9000, Belgium; INRA-UMR CARRTEL, Thonon les Bains, 74200, France; NatureMetrics Ltd, Egham, TW20 9TY, United Kingdom; Swedish University of Agricultural Sciences, Department of Aquatic Sciences and Assessment, PO Box 7050, Uppsala, SE-750 07, Sweden; University of Hull, Evolutionary Biology Group, School of Environmental SciencesHull HU6 7RX, United Kingdom; University of Duisburg-Essen, Aquatic Ecosystem Research, Essen, 45117, Germany; Finnish Environment Institute, Freshwater Centre, Jyväskylä, FI-40500, Finland; Botanic Garden Meise, Nieuwelaan, 38, Meise, 1860, Belgium; Royal Museum for Central Africa, Leuvensesteenweg, 13, Tervuren, 3080, Belgium; Agence Française pour la Biodiversité (AFB), 5 square Felix Nadar, Vincennes, 94300, France; Forest Research Center, School of Agriculture, University of Lisbon, Tapada da Ajuda, Lisbon, 1349-017, Portugal; Department of Life Sciences, Imperial College London, and Natural History Museum, Cromwell Road, London, SW7 5BD, United Kingdom; Bowburn Consultancy, 11 Monteigne Drive, Bowburn, Durham, DH6 5QB, United Kingdom

Recommended Citation:
Hering D.,Borja A.,Jones J.I.,et al. Implementation options for DNA-based identification into ecological status assessment under the European Water Framework Directive[J]. Water Research,2018-01-01,138
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