DOI: 10.1016/j.watres.2018.03.025
Scopus记录号: 2-s2.0-85044101612
论文题名: Emerging contaminants and nutrients synergistically affect the spread of class 1 integron-integrase (intI1) and sul1 genes within stable streambed bacterial communities
作者: Subirats J. ; Timoner X. ; Sànchez-Melsió A. ; Balcázar J.L. ; Acuña V. ; Sabater S. ; Borrego C.M.
刊名: Water Research
ISSN: 431354
出版年: 2018
卷: 138 起始页码: 77
结束页码: 85
语种: 英语
英文关键词: Antibiotic resistance genes
; Artificial streams
; Combined stress
; Emerging contaminants
; Long-term exposure
; Nutrients
; Streambed biofilms
Scopus关键词: Antibiotics
; Bacteria
; Biofilms
; Contamination
; Effluents
; Eutrophication
; Gene encoding
; Nutrients
; Sulfur compounds
; Antibiotic resistance genes
; Artificial streams
; Combined stress
; Emerging contaminant
; Long term exposure
; Streambeds
; River pollution
; activated carbon
; ammonia
; antibiotic agent
; ciprofloxacin
; diclofenac
; dissolved oxygen
; erythromycin
; integrase
; methyl paraben
; nitrate
; phosphate
; sulfamethoxazole
; 4 hydroxybenzoic acid ester
; antiinfective agent
; bacterial protein
; integron integrase IntI1
; abundance
; anthropogenic effect
; antibiotics
; aquatic organism
; biofilm
; chemical pollutant
; community composition
; gene
; gene expression
; microbial community
; nutrient dynamics
; pollution effect
; stream bed
; wastewater
; wastewater treatment
; Acetobacteraceae
; antibiotic resistance
; Article
; bacterial gene
; biofilm
; Bradyrhizobiaceae
; conductance
; cyanobacterium
; effluent
; Gammaproteobacteria
; gene
; integron
; intI1 gene
; microbial community
; nonhuman
; nutrient
; nutrient concentration
; pH
; priority journal
; sul1 gene
; Verrucomicrobia
; waste water
; bacterium
; genetics
; microbiology
; river
; water pollutant
; Bacteria (microorganisms)
; Anti-Bacterial Agents
; Bacteria
; Bacterial Proteins
; Ciprofloxacin
; Diclofenac
; Drug Resistance, Microbial
; Erythromycin
; Genes, Bacterial
; Integrases
; Parabens
; Rivers
; Sulfamethoxazole
; Waste Water
; Water Pollutants
英文摘要: Wastewater effluents increase the nutrient load of receiving streams while introducing a myriad of anthropogenic chemical pollutants that challenge the resident aquatic (micro)biota. Disentangling the effects of both kind of stressors and their potential interaction on the dissemination of antibiotic resistance genes in bacterial communities requires highly controlled manipulative experiments. In this work, we investigated the effects of a combined regime of nutrients (at low, medium and high concentrations) and a mixture of emerging contaminants (ciprofloxacin, erythromycin, sulfamethoxazole, diclofenac, and methylparaben) on the bacterial composition, abundance and antibiotic resistance profile of biofilms grown in artificial streams. In particular, we investigated the effect of this combined stress on genes encoding resistance to ciprofloxacin (qnrS), erythromycin (ermB), sulfamethoxazole (sul1 and sul2) as well as the class 1 integron-integrase gene (intI1). Only genes conferring resistance to sulfonamides (sul1 and sul2) and intI1 gene were detected in all treatments during the study period. Besides, bacterial communities exposed to emerging contaminants showed higher copy numbers of sul1 and intI1 genes than those not exposed, whereas nutrient amendments did not affect their abundance. However, bacterial communities exposed to both emerging contaminants and a high nutrient concentration (1, 25 and 1 mg L−1 of phosphate, nitrate and ammonium, respectively) showed the highest increase on the abundance of sul1 and intI1 genes thus suggesting a factors synergistic effect of both stressors. Since none of the treatments caused a significant change on the composition of bacterial communities, the enrichment of sul1 and intI1 genes within the community was caused by their dissemination under the combined pressure exerted by nutrients and emerging contaminants. To the best of our knowledge, this is the first study demonstrating the contribution of nutrients on the maintenance and spread of antibiotic resistance genes in streambed biofilms under controlled conditions. Our results also highlight that nutrients could enhance the effect of emerging contaminants on the dissemination of antibiotic resistance. © 2018 Elsevier Ltd Citation statistics:
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/112788
Appears in Collections: 气候减缓与适应
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作者单位: Catalan Institute for Water Research (ICRA), Scientific and Technological Park of the University of Girona, Girona, Spain; GRECO, Institute of Aquatic Ecology, University of Girona, Girona, Spain; Group of Molecular Microbial Ecology, Institute of Aquatic Ecology, University of Girona, Girona, Spain
Recommended Citation:
Subirats J.,Timoner X.,Sànchez-Melsió A.,et al. Emerging contaminants and nutrients synergistically affect the spread of class 1 integron-integrase (intI1) and sul1 genes within stable streambed bacterial communities[J]. Water Research,2018-01-01,138