DOI: 10.1016/j.watres.2018.02.001
Scopus记录号: 2-s2.0-85041390656
论文题名: Performance and genome-centric metagenomics of thermophilic single and two-stage anaerobic digesters treating cheese wastes
作者: Fontana A. ; Campanaro S. ; Treu L. ; Kougias P.G. ; Cappa F. ; Morelli L. ; Angelidaki I.
刊名: Water Research
ISSN: 431354
出版年: 2018
卷: 134 起始页码: 181
结束页码: 191
语种: 英语
英文关键词: Acidogenesis
; Dairy wastes
; Metagenomics
; Methanogens
; Syntrophisms
; Thermophilic anaerobic digestion
Scopus关键词: Agricultural wastes
; Biodiversity
; Genes
; Methane
; Methanogens
; Acidogenesis
; Dairy wastes
; Metagenomics
; Syntrophisms
; Thermophilic anaerobic digestion
; Anaerobic digestion
; acetic acid
; genomic DNA
; lactose
; methane
; anaerobic digestion
; biodegradation
; bioreactor
; community structure
; genome
; genomics
; methanogenic bacterium
; microbial community
; microbial ecology
; performance assessment
; retention
; thermophilic bacterium
; waste treatment
; wastewater treatment
; acidification
; anaerobic digestion
; Article
; bacterial genome
; bacterium
; Bifidobacterium
; Bifidobacterium crudilactis uc0001
; cheese
; community structure
; controlled study
; metagenomics
; microbial community
; microbial diversity
; nonhuman
; priority journal
; retention time
; Tepidanaerobacter acetatoxydans uc0018
; waste water management
; anaerobic growth
; bioreactor
; genetics
; metabolism
; metagenomics
; microbiology
; waste water
; Bifidobacterium
; Anaerobiosis
; Bacteria
; Bioreactors
; Cheese
; Metagenomics
; Methane
; Waste Water
英文摘要: The present research is the first comprehensive study regarding the thermophilic anaerobic degradation of cheese wastewater, which combines the evaluation of different reactor configurations (i.e. single and two-stage continuous stirred tank reactors) on the process efficiency and the in-depth characterization of the microbial community structure using genome-centric metagenomics. Both reactor configurations showed acidification problems under the tested organic loading rates (OLRs) of 3.6 and 2.4 g COD/L-reactor day and the hydraulic retention time (HRT) of 15 days. However, the two-stage design reached a methane yield equal to 95% of the theoretical value, in contrast with the single stage configuration, which reached a maximum of 33% of the theoretical methane yield. The metagenomic analysis identified 22 new population genomes and revealed that the microbial compositions between the two configurations were remarkably different, demonstrating a higher methanogenic biodiversity in the two-stage configuration. In fact, the acidogenic reactor of the serial configuration was almost solely composed by the lactose degrader Bifidobacterium crudilactis UC0001. The predictive functional analyses of the main population genomes highlighted specific metabolic pathways responsible for the AD process and the mechanisms of main intermediates production. Particularly, the acetate accumulation experienced by the single stage configuration was mainly correlated to the low abundant syntrophic acetate oxidizer Tepidanaerobacter acetatoxydans UC0018 and to the absence of aceticlastic methanogens. © 2018 The Authors Citation statistics:
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/112898
Appears in Collections: 气候减缓与适应
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作者单位: Department for Sustainable Food Process – DiSTAS, Catholic University of the Sacred Heart, Piacenza, 29122, Italy; Department of Biology, University of Padua, Padua, 35131, Italy; Department of Environmental Engineering, Technical University of Denmark, Kongens Lyngby, 2800, Denmark
Recommended Citation:
Fontana A.,Campanaro S.,Treu L.,et al. Performance and genome-centric metagenomics of thermophilic single and two-stage anaerobic digesters treating cheese wastes[J]. Water Research,2018-01-01,134