DOI: 10.1016/j.watres.2018.01.018
Scopus记录号: 2-s2.0-85040369923
论文题名: Estrogenic activity of cylindrospermopsin and anatoxin-a and their oxidative products by FeIII-B*/H2O2
作者: Liu J. ; Hernández S.E. ; Swift S. ; Singhal N.
刊名: Water Research
ISSN: 431354
出版年: 2018
卷: 132 起始页码: 309
结束页码: 319
语种: 英语
英文关键词: Cyanotoxins
; Degradation products
; Endocrine disrupting chemical
; Estrogenicity
; FeIII-TAML
; Modulation
Scopus关键词: Assays
; Binding energy
; Bins
; Catalyst activity
; Catalysts
; Chemicals
; Degradation
; Drug products
; Mass spectrometry
; Modulation
; Oxidation
; Cyanobacterial blooms
; Cyanotoxins
; Degradation products
; Endocrine disrupting chemicals
; Estrogenic activities
; Estrogenicity
; Tandem mass spectrometry
; Yeast estrogen screen assays
; Endocrine disrupters
; anatoxin a
; cylindrospermopsin
; endocrine disruptor
; estradiol
; estrogen
; estrogen receptor
; ferrous ion
; hydrogen peroxide
; algal bloom
; bioassay
; catalyst
; concentration (composition)
; cyanobacterium
; degradation
; endocrine disruptor
; estrogenic compound
; iron
; oxidation
; oxidative stress
; toxin
; Article
; catalyst
; cytotoxicity
; liquid chromatography-mass spectrometry
; nonhuman
; oxidation
; priority journal
; yeast cell
; Animalia
; Cyanobacteria
英文摘要: The cyanotoxins released into waters during cyanobacterial blooms can pose serious hazards to humans and animals. Apart from their toxicological mechanisms, cyanotoxins have been shown to be involved in estrogenic activity by in vivo and in vitro assays; however, there is limited information on the change in estrogenicity of cyanotoxins following chemical oxidation. In this study, the estrogenic activity of cylindrospermopsin (CYL) and anatoxin-a (ANA) at concentrations ranging from 2.4 × 10−7 M to 2.4 × 10−12 M (CYL) and 7.1 × 10−6 M to 7.1 × 10−11 M (ANA), and after treatment by the FeIII-B*/H2O2 catalyst system, was investigated by the yeast estrogen screen (YES) assay. The results indicate that CYL and ANA acted as agonists in the YES assay (CYL logEC50 = −8.901; ANA logEC50 = −6.789), their binding affinity to estrogen receptors is associated with their intrinsic properties, including ring structures and toxicant properties. CYL and ANA were shown to simulate endocrine disrupting chemicals (EDCs) to modulate the 17β-estradiol-induced estrogenic activity, resulting in non-monotonic dose responses. The treated CYL showed a significantly altered estrogenicity compared to the untreated CYL (T(2) = 8.168, p ≤.05), while the estrogenicity of the treated ANA was not significantly different to the untreated ANA (T(2) = 1.295, p >.05). Intermediate products generated from CYL and ANA oxidized by FeIII-B*/H2O2 were identified using Q-Exactive Tandem Mass Spectrometry (LC-MS/MS). Treatment with FeIII-B*/H2O2 yielded open-ring by-products which likely resulted in CYL's reduced binding affinity to estrogen receptors. The insignificant change in the estrogenicity of treated ANA was possibly a result of its multiple ring structure products, which were likely able to bind to estrogen receptors. The comparisons for the estrogenicity of these cyanotoxins before and after FeIII-B*/H2O2 treatment suggest that the reductions in estrogenicity achieved by oxidation were dependent on the levels of cyanotoxins removed, as well as the estrogenicity of the degradation products. This is the first study on the change in the estrogenicity of CYL and ANA upon oxidation by FeIII-B*/H2O2, a high activity catalyst system. © 2018 Elsevier Ltd Citation statistics:
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/112980
Appears in Collections: 气候减缓与适应
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作者单位: Department of Civil & Environmental Engineering, The University of Auckland, Auckland, 1142, New Zealand; Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Autonoma de Mexico, Ciudad de Mexico, Mexico; Molecular Medicine and Pathology, The University of Auckland, Auckland, 1142, New Zealand
Recommended Citation:
Liu J.,Hernández S.E.,Swift S.,et al. Estrogenic activity of cylindrospermopsin and anatoxin-a and their oxidative products by FeIII-B*/H2O2[J]. Water Research,2018-01-01,132