DOI: 10.1016/j.watres.2017.12.040
Scopus记录号: 2-s2.0-85040131007
论文题名: Microbial community of a gasworks aquifer and identification of nitrate-reducing Azoarcus and Georgfuchsia as key players in BTEX degradation
作者: Sperfeld M. ; Rauschenbach C. ; Diekert G. ; Studenik S.
刊名: Water Research
ISSN: 431354
出版年: 2018
卷: 132 起始页码: 146
结束页码: 157
语种: 英语
英文关键词: Benzoyl-CoA reductase
; Bioremediation
; Box pathway
; Environmental pollutions
; Functional gene marker
; Microbial communities
Scopus关键词: Aquifers
; Biodegradation
; Bioremediation
; Coal tar
; Genes
; Groundwater resources
; Microorganisms
; Nitrates
; RNA
; Sulfur compounds
; Xylene
; Benzoyl-CoA reductase
; Box pathway
; Environmental pollutions
; Functional genes
; Microbial communities
; Groundwater pollution
; aromatic compound
; benzene
; ethylbenzene
; genomic DNA
; ground water
; meta xylene
; ortho xylene
; oxidoreductase
; para xylene
; RNA 16S
; toluene
; xylene
; 4-xylene
; benzene
; benzene derivative
; coal tar
; ethylbenzene
; nitric acid derivative
; sulfate
; toluene
; xylene
; abundance
; anoxic conditions
; aquifer
; aquifer pollution
; bacterium
; biodegradation
; biomarker
; bioremediation
; BTEX
; coal tar
; enzyme
; enzyme activity
; genetic analysis
; groundwater
; groundwater pollution
; growth
; inoculation
; microbial community
; amplicon
; anaerobic digestion
; aquifer
; Article
; Azoarcus
; bacterium identification
; bamA gene
; Betaproteobacteria
; bioremediation
; controlled study
; DNA extraction
; enrichment culture
; gene cluster
; gene sequence
; Georgfuchsia toluolica
; marker gene
; microbial community
; microbial degradation
; nitrate reducing bacterium
; nonhuman
; open reading frame
; pollution
; priority journal
; Sanger sequencing
; genetics
; Germany
; metabolism
; microbiology
; water pollutant
; Germany
; Thuringia
; Azoarcus
; Betaproteobacteria
; Deltaproteobacteria
; Firmicutes
; Pelotomaculum
; Rhodoferax
; Azoarcus
; Benzene
; Benzene Derivatives
; Betaproteobacteria
; Biodegradation, Environmental
; Coal Tar
; Germany
; Groundwater
; Nitrates
; RNA, Ribosomal, 16S
; Sulfates
; Toluene
; Water Pollutants, Chemical
; Xylenes
英文摘要: We analyzed a coal tar polluted aquifer of a former gasworks site in Thuringia (Germany) for the presence and function of aromatic compound-degrading bacteria (ACDB) by 16S rRNA Illumina sequencing, bamA clone library sequencing and cultivation attempts. The relative abundance of ACDB was highest close to the source of contamination. Up to 44% of total 16S rRNA sequences were affiliated to ACDB including genera such as Azoarcus, Georgfuchsia, Rhodoferax, Sulfuritalea (all Betaproteobacteria) and Pelotomaculum (Firmicutes). Sequencing of bamA, a functional gene marker for the anaerobic benzoyl-CoA pathway, allowed further insights into electron-accepting processes in the aquifer: bamA sequences of mainly nitrate-reducing Betaproteobacteria were abundant in all groundwater samples, whereas an additional sulfate-reducing and/or fermenting microbial community (Deltaproteobacteria, Firmicutes) was restricted to a highly contaminated, sulfate-depleted groundwater sampling well. By conducting growth experiments with groundwater as inoculum and nitrate as electron acceptor, organisms related to Azoarcus spp. were identified as key players in the degradation of toluene and ethylbenzene. An organism highly related to Georgfuchsia toluolica G5G6 was enriched with p-xylene, a particularly recalcitrant compound. The anaerobic degradation of p-xylene requires a metabolic trait that was not described for members of the genus Georgfuchsia before. In line with this, we were able to identify a putative 4-methylbenzoyl-CoA reductase gene cluster in the respective enrichment culture, which is possibly involved in the anaerobic degradation of p-xylene. © 2017 Elsevier Ltd Citation statistics:
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/112986
Appears in Collections: 气候减缓与适应
There are no files associated with this item.
作者单位: Institute of Microbiology, Friedrich Schiller University Jena, Department of Applied and Ecological Microbiology, Philosophenweg 12, Jena, 07743, Germany; JENA-GEOS®-Ingenieurbüro GmbH, Saalbahnhofstraße 25c, Jena, 07743, Germany
Recommended Citation:
Sperfeld M.,Rauschenbach C.,Diekert G.,et al. Microbial community of a gasworks aquifer and identification of nitrate-reducing Azoarcus and Georgfuchsia as key players in BTEX degradation[J]. Water Research,2018-01-01,132