DOI: 10.1016/j.watres.2017.10.070
Scopus记录号: 2-s2.0-85036518519
论文题名: Responses of riverbed sediment bacteria to heavy metals: Integrated evaluation based on bacterial density, activity and community structure under well-controlled sequencing batch incubation conditions
作者: Du H. ; Harata N. ; Li F.
刊名: Water Research
ISSN: 431354
出版年: 2018
卷: 130 起始页码: 115
结束页码: 126
语种: 英语
英文关键词: 16S rDNA
; Bacterial community
; Heavy metals
; River sediment
; Sediment bacteria
; Toxicity
Scopus关键词: Catchments
; Glucose
; Heavy metals
; Polymerase chain reaction
; River pollution
; Rivers
; Sediments
; Toxicity
; 16s rDNA
; Bacterial community
; Community structures
; Concentration levels
; Controlled conditions
; Heterotrophic bacterias
; Integrated evaluation
; River sediments
; Bacteria
; cadmium
; chromium
; copper
; DNA 16S
; glucose
; heavy metal
; lead
; heavy metal
; bacterium
; bioindicator
; catchment
; community response
; community structure
; DNA
; fluvial deposit
; glucose
; heavy metal
; inhibition
; integrated approach
; land cover
; land use
; microbial activity
; microbial community
; pollution tolerance
; population density
; river bed
; river water
; toxicity
; trend analysis
; valuation
; water pollution
; Article
; carbon source
; community structure
; concentration (parameters)
; controlled study
; density
; land use
; microbial activity
; microbial community
; nonhuman
; polymerase chain reaction
; priority journal
; river
; sediment
; sequence analysis
; suspension
; bacterium
; chemistry
; China
; classification
; drug effect
; environmental monitoring
; growth, development and aging
; microbiology
; procedures
; water pollutant
; Bacteria (microorganisms)
; Bacteria
; China
; Environmental Monitoring
; Geologic Sediments
; Metals, Heavy
; Rivers
; Water Pollutants, Chemical
英文摘要: Better understanding the responses of riverbed sediment bacteria to heavy metals is a key for considering using riverbed sediment bacterial community as an indicator of river water contamination by heavy metals. For this, integrated evaluation based on bacterial density, activity and community structure through incubation experiments under well-controlled conditions is necessary to obtain more closely relevant findings that are difficult to achieve through field studies. The findings may also include those that can serve as new evidence to clarify contrary findings reported in previous studies. In this study, sequencing batch incubation experiments were performed using sediment suspensions from three rivers with catchment of different land covers and uses. The effects of four metals (Pb, Cr, Cd and Cu) were investigated by spiking them separately under three different concentration levels. Glucose was added once every day as the carbon source throughout the whole incubation lasted for 30 days. The changing trends of the density of general and heterotrophic bacteria showed clearly that the responses of sediment bacteria to Cu were obviously stronger, followed by that to Cd; whereas, the responses to Pb and Cr were not apparent. For incubation with Cu, a short-term inhibition effect appeared in the initial 3 days and was then followed by a long-term promotion effect reflected by obvious increases of bacterial density against control. In regard of bacterial activity evaluated based on the first-order consumption rate for glucose, a trend of decreases was revealed. The results of PCR-DGGE and sequence analysis of extracted 16S rDNA further suggested there were bacterial species that had strong tolerance against the metal and could grow readily to become new dominating ones. The existence of such bacterial species was inferred as the reason leading to the observed increases of bacterial density during incubation with the metal. © 2017 Citation statistics:
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/113047
Appears in Collections: 气候减缓与适应
There are no files associated with this item.
作者单位: College of Urban Construction, Nanjing Tech University, PuZhu Road, Nanjing, China; Division of Environmental Engineering, Ichikawa Koumuten Co., Ltd, Gifu, Japan; River Basin Research Center, Gifu University, 1-1 Yanagido, Gifu, Japan
Recommended Citation:
Du H.,Harata N.,Li F.. Responses of riverbed sediment bacteria to heavy metals: Integrated evaluation based on bacterial density, activity and community structure under well-controlled sequencing batch incubation conditions[J]. Water Research,2018-01-01,130