DOI: 10.1016/j.watres.2017.11.020
Scopus记录号: 2-s2.0-85034852643
论文题名: An improved protocol for extracting extracellular polymeric substances from granular filter media
作者: Keithley S.E. ; Kirisits M.J.
刊名: Water Research
ISSN: 431354
出版年: 2018
卷: 129 起始页码: 419
结束页码: 427
语种: 英语
英文关键词: Backwash
; Drinking-water biofilters
; Extracellular polymeric substances
; Extraction
Scopus关键词: Activated carbon
; Activated carbon treatment
; Anthracite
; Bandpass filters
; Biofilms
; Biofilters
; Body fluids
; Potable water
; Proteins
; Sodium chloride
; Backwash
; Bovine serum albumins
; Extra-cellular polymeric substances
; Extraction protocols
; Full-scale biofilter
; Granular activated carbons
; Physical treatments
; Protein concentrations
; Extraction
; activated carbon
; bovine serum albumin
; buffer
; drinking water
; edetic acid
; extracellular polymeric substance
; glucose
; polymer
; polysaccharide
; protein
; unclassified drug
; charcoal
; polymer
; polysaccharide
; activated carbon
; backwater
; biofilm
; concentration (composition)
; drinking water
; extraction method
; filter
; polymer
; water treatment
; Article
; biofilm
; biomass
; extraction
; nonhuman
; physical parameters
; priority journal
; sand
; temperature
; chemistry
; comparative study
; devices
; procedures
; water management
; Canada
; United States
; Bovinae
; Biofilms
; Charcoal
; Drinking Water
; Polymers
; Polysaccharides
; Temperature
; Water Purification
英文摘要: Extracellular polymeric substances (EPS) are an important parameter in drinking-water biofilters, and, to date, this is the first study to compare protocols from the literature for extracting EPS from granular filter media. Five extraction protocols were compared, and one was improved by varying the type of initial physical treatment and the shaking intensity, temperature, and time of incubation. Extracting EPS from granular filter media in triplicate by combining 2 g (wet weight) of filter media with 10 mL of extraction buffer (10 mM Tris, 10 mM EDTA, 2.5% NaCl, pH 8), vortexing for 1 min, and incubating for 4 h at 35 °C with shaking at 200 rpm yielded significantly higher EPS polysaccharide and/or protein concentrations than did protocols from the literature. This improved protocol extracted a significant fraction of the biofilm attached to sand but was less effective on biofilm attached to anthracite or granular activated carbon (GAC). A survey of 11 full-scale biofilters from the U.S. and Canada revealed that EPS polysaccharide and protein concentrations can vary over one order of magnitude, from 0.02 to 0.60 mg glucose/g total solids (TS) and 0.27 to 3.38 mg bovine serum albumin/g TS, respectively. Backwashing significantly lowered the biomass and EPS polysaccharide concentrations, but the majority of the biofilm remained attached to the filter media after backwashing, as expected. The fraction of EPS polysaccharides and proteins removed during backwashing did not differ between anthracite and GAC biofilters. The improved EPS extraction protocol can be utilized to investigate the role of EPS in biofilter performance. © 2017 Elsevier Ltd Citation statistics:
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/113098
Appears in Collections: 气候减缓与适应
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作者单位: Department of Civil, Architectural, and Environmental Engineering, The University of Texas at Austin, 301 E. Dean Keeton St., Stop 1700, Austin, TX 78712, United States; Tighe & Bond, 1 University Avenue, Suite 104, Westwood, MA 02090, United States
Recommended Citation:
Keithley S.E.,Kirisits M.J.. An improved protocol for extracting extracellular polymeric substances from granular filter media[J]. Water Research,2018-01-01,129