DOI: 10.1016/j.watres.2017.10.071
Scopus记录号: 2-s2.0-85032807696
论文题名: A human fecal contamination score for ranking recreational sites using the HF183/BacR287 quantitative real-time PCR method
作者: Cao Y. ; Sivaganesan M. ; Kelty C.A. ; Wang D. ; Boehm A.B. ; Griffith J.F. ; Weisberg S.B. ; Shanks O.C.
刊名: Water Research
ISSN: 431354
出版年: 2018
卷: 128 起始页码: 148
结束页码: 156
语种: 英语
英文关键词: Human fecal pollution
; Microbial source tracking
; qPCR
; Site prioritization
Scopus关键词: Biomarkers
; Contamination
; Water pollution
; Water quality
; Analysis strategies
; Field implementation
; Human fecal pollution
; Microbial source tracking
; Prioritization
; qPCR
; Quantitative real time PCR
; Recreational water
; Polymerase chain reaction
; Bayesian analysis
; feces
; microbial activity
; polymerase chain reaction
; prioritization
; public health
; quantitative analysis
; recreational activity
; source identification
; water quality
; Article
; Bayes theorem
; human
; human fecal contamination score
; priority journal
; quantitative analysis
; real time polymerase chain reaction
; reliability
; waste management
; water sampling
; California
; environmental monitoring
; feces
; microbiology
; procedures
; quality control
; real time polymerase chain reaction
; recreation
; reproducibility
; standards
; water quality
; Bayes Theorem
; California
; Environmental Monitoring
; Feces
; Humans
; Quality Control
; Real-Time Polymerase Chain Reaction
; Recreation
; Reproducibility of Results
; Water Microbiology
; Water Quality
英文摘要: Human fecal pollution of recreational waters remains a public health concern worldwide. As a result, there is a growing interest in the application of human-associated fecal source identification quantitative real-time PCR (qPCR) technologies for water quality research and management. However, there are currently no standardized approaches for field implementation and interpretation of qPCR data. In this study, a standardized HF183/BacR287 qPCR method was combined with a water sampling strategy and a novel Bayesian weighted average approach to establish a human fecal contamination score (HFS) that can be used to prioritize sampling sites for remediation based on measured human waste levels. The HFS was then used to investigate 975 study design scenarios utilizing different combinations of sites with varying sampling intensities (daily to once per week) and number of qPCR replicates per sample (2–14 replicates). Findings demonstrate that site prioritization with HFS is feasible and that both sampling intensity and number of qPCR replicates influence reliability of HFS estimates. The novel data analysis strategy presented here provides a prescribed approach for the implementation and interpretation of human-associated HF183/BacR287 qPCR data with the goal of site prioritization based on human fecal pollution levels. In addition, information is provided for future users to customize study designs for optimal HFS performance. © 2017 Citation statistics:
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/113140
Appears in Collections: 气候减缓与适应
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作者单位: Southern California Coastal Water Research Project Authority, Costa Mesa, CA 92626, United States; U.S. Environmental Protection Agency, Office of Research and Development, Cincinnati, OH 45268, United States; Department of Civil and Environmental Engineering, Stanford University, Stanford, CA 94305, United States
Recommended Citation:
Cao Y.,Sivaganesan M.,Kelty C.A.,et al. A human fecal contamination score for ranking recreational sites using the HF183/BacR287 quantitative real-time PCR method[J]. Water Research,2018-01-01,128