DOI: 10.1016/j.watres.2017.10.046
Scopus记录号: 2-s2.0-85032686151
论文题名: Polyhydroxyalkanoates (PHA) production from fermented crude glycerol: Study on the conversion of 1,3-propanediol to PHA in mixed microbial consortia
作者: Burniol-Figols A. ; Varrone C. ; Daugaard A.E. ; Le S.B. ; Skiadas I.V. ; Gavala H.N.
刊名: Water Research
ISSN: 431354
出版年: 2018
卷: 128 起始页码: 255
结束页码: 266
语种: 英语
英文关键词: 1,3-Propanediol
; Butyrate
; Crude glycerol
; Mixed microbial consortia
; PHA
; Polyhydroxyalkanoates
Scopus关键词: Carboxylation
; Effluents
; Food supply
; Glycerol
; Nitrogen
; Substrates
; 1 ,3 propanediol
; Butyrate
; Crude glycerol
; Microbial consortia
; Polyhydroxyalkanoates
; Volatile fatty acids
; 1,3 propanediol
; glycerol derivative
; nitrogen derivative
; polyhydroxyalkanoic acid
; 1,3-propanediol
; glycerol
; glycogen
; nitrogen
; polyhydroxyalkanoic acid
; propanediol derivative
; propylene glycol
; volatile fatty acid
; alcohol
; enrichment
; ester
; fatty acid
; fermentation
; microbial activity
; nitrogen
; polymer
; substrate
; Article
; controlled study
; decarboxylation
; effluent
; fermentation
; microbial consortium
; nitrogen availability
; nonhuman
; priority journal
; bioreactor
; biosynthesis
; evaluation study
; metabolism
; microbial consortium
; Bioreactors
; Fatty Acids, Volatile
; Fermentation
; Glycerol
; Glycogen
; Microbial Consortia
; Nitrogen
; Polyhydroxyalkanoates
; Propylene Glycol
; Propylene Glycols
英文摘要: Crude glycerol, a by-product from the biodiesel industry, can be converted by mixed microbial consortia into 1,3-propanediol (1,3-PDO) and volatile fatty acids. In this study, further conversion of these main products into polyhydroxyalkanoates (PHA) was investigated with the focus on 1,3-PDO. Two different approaches for the enrichment of PHA accumulating microbial consortia using an aerobic dynamic feeding strategy were applied. With the first approach, where nitrogen was present during the whole cycle, no net production of PHA from 1,3-PDO was observed in the fermented effluent, not even in a nitrogen-limited PHA accumulation assay. Nevertheless, experiments in synthetic substrates revealed that the conversion of 1,3-PDO to PHA was possible under nitrogen limiting conditions. Thus, a different enrichment strategy was formulated where nitrogen was limited during the feast phase to stimulate the storage response. Nitrogen was still supplied during the famine phase. With the latter strategy, a net production of PHA from 1,3-PDO was observed at a yield of 0.24 Cmol PHA/Cmol 1,3-PDO. The overall yield from the fermented effluent was 0.42 Cmol PHA/Cmol substrate. Overall, the PHA yield from 1,3-PDO seemed to be limited, similarly to when using glycerol as a substrate, by a decarboxylation step and accumulation of other storage polymers such as glycogen, and possibly, lipid inclusions. © 2017 Elsevier Ltd Citation statistics:
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/113146
Appears in Collections: 气候减缓与适应
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作者单位: Technical University of Denmark (DTU), Dept. of Chemical and Biochemical Engineering, Center for Bioprocess Engineering, Søltofts Plads, Building 229, Kgs. Lyngby, 2800, Denmark; Technical University of Denmark (DTU), Dept. of Chemical and Biochemical Engineering, Danish Polymer Center, Søltofts Plads, Building 229, Kgs. Lyngby, 2800, Denmark; Technical University of Denmark (DTU), Dept. of Chemical and Biochemical Engineering, Pilot Plant, Søltofts Plads, Building 229, Kgs. Lyngby, 2800, Denmark; SINTEF, Materials and Chemistry, Dept. Biotechnology and Nanomedicine, Postboks 4760 Sluppen, Trondheim, 7465, Norway
Recommended Citation:
Burniol-Figols A.,Varrone C.,Daugaard A.E.,et al. Polyhydroxyalkanoates (PHA) production from fermented crude glycerol: Study on the conversion of 1,3-propanediol to PHA in mixed microbial consortia[J]. Water Research,2018-01-01,128