DOI: 10.1016/j.watres.2017.10.030
Scopus记录号: 2-s2.0-85031994366
论文题名: Bluephage: A rapid method for the detection of somatic coliphages used as indicators of fecal pollution in water
作者: Muniesa M. ; Ballesté E. ; Imamovic L. ; Pascual-Benito M. ; Toribio-Avedillo D. ; Lucena F. ; Blanch A.R. ; Jofre J.
刊名: Water Research
ISSN: 431354
出版年: 2018
卷: 128 起始页码: 10
结束页码: 19
语种: 英语
英文关键词: Coliphages
; Fecal pollution
; Indicator
; New tools
; Virus
Scopus关键词: Bacteriophages
; Cost effectiveness
; Encoding (symbols)
; Enzymes
; Escherichia coli
; Glucose
; Indicators (instruments)
; Pollution detection
; Viruses
; Water management
; Beta-glucuronidase
; Coliphages
; Culturable microorganisms
; Fecal pollution
; Microbiological methods
; Reproducibilities
; Somatic coliphages
; Water management policy
; Water pollution
; beta glucuronidase
; glucuronic acid
; oligonucleotide
; protein C
; bioindicator
; coliform bacterium
; detection method
; enzyme activity
; gene expression
; quantitative analysis
; virus
; water management
; water pollution
; Article
; bacterial cell
; bacterium detection
; bioaccumulation
; bluephage
; coliphage
; color
; cytolysis
; Escherichia coli
; false negative result
; fecal pollution
; feces
; gene
; gene overexpression
; microbiological examination
; nonhuman
; priority journal
; quantitative analysis
; uida gene
; uidb gene
; uidc gene
; water management
; water pollution
; analysis
; coliphage
; feces
; isolation and purification
; microbiology
; reproducibility
; water pollution
; water quality
; Bacteria (microorganisms)
; Escherichia coli
; Coliphages
; Escherichia coli
; Feces
; Reproducibility of Results
; Water Microbiology
; Water Pollution
; Water Quality
英文摘要: The use of somatic coliphages as indicators of fecal and viral pollution in water and food has great potential due to the reliability, reproducibility, speed and cost effectiveness of methods for their detection. Indeed, several countries already use this approach in their water management policies. Although standardized protocols for somatic coliphage detection are available, user-friendly commercial kits would facilitate their routine implementation in laboratories. The new method presented here allows detection of up to 1 somatic coliphage in under 3.5 h, well within one working day. The method is based on a modified Escherichia coli strain with knocked-out uidB and uidC genes, which encode the transport of glucuronic acid inside cells, and overexpressing uidA, which encodes the enzyme β-glucuronidase. The enzyme accumulated in the bacterial cells only has contact with its substrate after cell lysis, such as that caused by phages, since the strain cannot internalize the substrate. When the enzyme is released into the medium, which contains a chromogen analogous to glucuronic acid, it produces a change of color from yellow to dark blue. This microbiological method for the determination of fecal pollution via the detection of culturable microorganisms can be applied to diverse sample types and volumes for qualitative (presence/absence) and quantitative analysis and is the fastest reported to date. © 2017 Elsevier Ltd Citation statistics:
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/113147
Appears in Collections: 气候减缓与适应
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作者单位: Department of Genetics, Microbiology and Statistics, Microbiology Section, Faculty of Biology, University of Barcelona, Diagonal 643, Barcelona, E-08028, Spain
Recommended Citation:
Muniesa M.,Ballesté E.,Imamovic L.,et al. Bluephage: A rapid method for the detection of somatic coliphages used as indicators of fecal pollution in water[J]. Water Research,2018-01-01,128