DOI: 10.1016/j.watres.2017.10.060
Scopus记录号: 2-s2.0-85032796561
论文题名: Biohydrogen production at pH below 3.0: Is it possible?
作者: Mota V.T. ; Ferraz Júnior A.D.N. ; Trably E. ; Zaiat M.
刊名: Water Research
ISSN: 431354
出版年: 2018
卷: 128 起始页码: 350
结束页码: 361
语种: 英语
英文关键词: Acid-tolerant bacteria
; Acidogenic reactor
; Biohydrogen
; Dark fermentation
; Hydrogen
; pH
Scopus关键词: Fermentation
; Hydrogen
; pH
; Sugar (sucrose)
; Acid-tolerant bacteria
; Acidogenic reactor
; Bio-hydrogen
; Bio-hydrogen production
; Biological hydrogen production
; Biomass concentrations
; Dark fermentation
; Hydrogen production rate
; Photobiological hydrogen production
; acetic acid
; alcohol
; hydrogen
; ribosome DNA
; sucrose
; biofuel
; hydrogen
; bacterium
; biomass
; bioreactor
; effluent
; fermentation
; inhibition
; pH
; sucrose
; Article
; biomass
; controlled study
; dynamics
; fermentation
; high performance liquid chromatography
; metabolism
; microflora
; mixed cell culture
; neighbor joining method
; nucleotide sequence
; pH
; phylogenetic tree
; priority journal
; reactor design
; slaughterhouse
; suspended particulate matter
; temperature
; thermal conductivity
; turbidity
; waste water
; bioreactor
; evaluation study
; fermentation
; flocculation
; metabolism
; pH
; Clostridium
; Ethanoligenens
; Biofuels
; Biomass
; Bioreactors
; Ethanol
; Fermentation
; Flocculation
; Hydrogen
; Hydrogen-Ion Concentration
; Sucrose
英文摘要: Biological hydrogen production was investigated in continuous acidogenic reactors fed with sucrose at 30 °C without pH control. In the first experimental phase, three reactors were compared: a structured fixed-bed (FB), a granular UASB (UG) and a flocculent UASB (UF-1). They were run at 3.3 h HRT and 33 gCOD L−1d−1 OLR. Hydrogen production occurred throughout the experimental period with an average effluent pH of only 2.8. The FB, UG and UF-1 reactors presented volumetric hydrogen production rates (VHPR) of 95 ± 69, 45 ± 37 and 54 ± 32 mLH2 L−1h−1, respectively; and H2 yields (HY) of 1.5 ± 0.8, 0.8 ± 0.6 and 1.2 ± 0.7 molH2 mol−1 sucroseconsumed, respectively. The UF-1 reactor showed intermediate VHPR and HY, but no declining trend, contrary to what was observed in the FB reactor. Thus, aiming at continuous and long-term H2 production, a flocculent UASB was applied in the second experimental phase. In this phase, the HRT of the acidogenic reactor, which was named UF-2, was raised to 4.6 h, resulting in an OLR of 25 gCOD L−1d−1. The VHPR and the HY increased considerably to 175 ± 44 mLH2 L−1h−1 and 3.4 ± 0.7 molH2 mol−1 sucroseconsumed, respectively. These improvements were accompanied by greater sucrose removal, higher suspended biomass concentration, less production of lactate and more of acetate, and high ethanol concentration. Contradicting the current published literature data that reports strong inhibition of H2 production by dark fermentation at pH less than 4.0, the UF-2 reactor presented stable, long-term H2 production with satisfactory yields at pH 2.7 on average. 16 S rDNA sequencing revealed that two sequences assigned as Ethanoligenens and Clostridium accounted for over 70% of the microbiota in all the reactors. The non-necessity of adding alkalizing agents and the successful H2 production under very acid conditions, demonstrated in this study, open a new field of investigation in biological hydrogen production by dark fermentation towards a more sustainable and feasible technology. © 2017 Elsevier Ltd Citation statistics:
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/113163
Appears in Collections: 气候减缓与适应
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作者单位: LPB, Department of Hydraulics and Sanitary Engineering, São Carlos School of Engineering, University of São Paulo (USP), Avenida João Dagnone, 1100, São Carlos, SP 13563-120, Brazil; CTBE, CNPEM, Rua Giuseppe Máximo Scolfaro 10000, Campinas, SP 13083-970, Brazil; LBE, INRA, Univ Montpellier, Narbonne, 11100, France
Recommended Citation:
Mota V.T.,Ferraz Júnior A.D.N.,Trably E.,et al. Biohydrogen production at pH below 3.0: Is it possible?[J]. Water Research,2018-01-01,128