DOI: 10.1016/j.jcou.2018.11.007
Scopus记录号: 2-s2.0-85057584744
论文题名: Comparative effect of supercritical carbon dioxide and high pressure processing on structural changes and activity loss of oxidoreductive enzymes
作者: Marszałek K. ; Doesburg P. ; Starzonek S. ; Szczepańska J. ; Woźniak Ł. ; Lorenzo J.M. ; Skaopska S. ; Rzoska S. ; Barba F.J.
刊名: Journal of CO2 Utilization
ISSN: 22129820
出版年: 2019
卷: 29 起始页码: 46
结束页码: 56
语种: 英语
英文关键词: Conductivity
; CuCl2 crystallization with additives
; High pressure processing
; Kinetics of inactivation
; Peroxidase
; Polyphenol oxidase
; Supercritical-CO2
Scopus关键词: Additives
; Carbon dioxide
; Chlorine compounds
; Data handling
; Electric conductivity
; Enzymes
; High pressure effects
; Supercritical fluid extraction
; Electrical conductivity
; High pressure processing
; Horse-radish peroxidase
; Peroxidase
; Polyphenol oxidase
; Supercritical carbon dioxides
; Supercritical CO2
; Temperature and pressures
; Copper compounds
英文摘要: Due to the CO2 specific characteristics, it has been used as supercritical (Sc) fluid for several applications, including enzyme inactivation. The influence of Sc-CO2 (10-65 MPa/10-30 min/35-65 °C) on mushroom polyphenol oxidase (PPO) and horseradish peroxidase (POD) was evaluated and the results were compared with those found using high pressure processing (HPP) (200-900 MPa/5-45 °C/1-15 min). The free ion concentration was also studied to compare the enzymatic activity and changes in electrical conductivity. Additionally both enzymes, untreated or treated using either Sc-CO2 or HPP, were used as additives in the CuCl2 crystallization method. The resulting additive-specific CuCl2 patterns were characterized based on different structural features. Sc-CO2 was found to have a significant influence on PPO and POD activities, and an increased reduction in the residual activity of both enzymes was observed when the Sc-CO2 pressure was increased. However, PPO was more resistant to temperature and pressure than POD. The D-value calculated for POD was in the range of 38.3 and 592.0 min, and 60.6 and 291.5 min, for HPP and Sc-CO2 treatments, respectively; whereas for PPO varied from 103.1 to 284.3 min under HPP and from 83.4 to 303.0 min using the Sc-CO2 treatment. It can be concluded that the application of Sc-CO2 could be an effective tool for inactivating PPO and POD enzymes. Moreover, both enzymes and treatments could be differentiated significantly based on the resulting CuCl2 crystallization patterns. © 2018 Elsevier Ltd. All rights reserved. Citation statistics:
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/117327
Appears in Collections: 气候变化与战略
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Recommended Citation:
Marszałek K.,Doesburg P.,Starzonek S.,et al. Comparative effect of supercritical carbon dioxide and high pressure processing on structural changes and activity loss of oxidoreductive enzymes[J]. Journal of CO2 Utilization,2019-01-01,29