DOI: 10.1016/j.watres.2019.02.003
Scopus记录号: 2-s2.0-85062004335
论文题名: Evaluation of pepper mild mottle virus as an indicator of human faecal pollution in shellfish and growing waters
作者: Gyawali P. ; Croucher D. ; Ahmed W. ; Devane M. ; Hewitt J.
刊名: Water Research
ISSN: 431354
出版年: 2019
卷: 154 起始页码: 370
结束页码: 376
语种: 英语
英文关键词: Faecal contamination
; Indicator
; Microbial source tracking
; PMMoV
; Shellfish
; Shellfish growing water
英文摘要: Bivalve molluscan shellfish grown in areas impacted by human faecal pollution are at risk of being contaminated with multiple enteric viruses. To minimise the public health risks associated with shellfish consumption, determining the presence of faecal contamination in shellfish and their growing waters is crucial. In this study, we evaluated the use of pepper mild mottle virus (PMMoV) as an indicator of human faecal contamination in oysters, mussels, cockles and shellfish growing waters in New Zealand. Using reverse transcription quantitative polymerase chain reaction (RT-qPCR) the presence, and where applicable, the concentration of PMMoV was determined in faeces from 11 different animal species, influent (untreated) wastewater, shellfish and shellfish growing waters. Non-human faecal samples (from seagull, Canada goose, black swan and dog) were RT-qPCR positive for PMMoV. The faecal source specificity of PMMoV was 0.83 (maximum value of 1) when ‘detected but not quantifiable’ (DNQ) values were used. However, when ‘lower limit of quantification’ (LLOQ) values were used, the specificity increased to 0.92. The PMMoV concentration in influent wastewater (n = 10) ranged from 6.3 to 7.7 log 10 genome copies (GC)/L with a mean (±standard deviation) of 7.1 ± 0.5 log 10 GC/L. The overall occurrence of PMMoV in shellfish and shellfish growing waters from four different areas was 46/51 (90%) and 29/52 (56%), respectively. Of the cockles collected from an area known to be impacted by effluent wastewater, 14/14 (100%) contained PMMoV concentrations above the LLOQ. In contrast, only 13/37 (35%) shellfish and 6/52 (11.5%) growing water samples collected from three areas with low anthropogenic impact contained PMMoV concentrations above the LLOQ. The high concentration of PMMoV in influent wastewater indicates that PMMoV may be a promising indicator of human faecal contamination. The presence of PMMoV in shellfish and growing waters with a low anthropogenic impact may be of avian origin, and this needs to be considered if using PMMoV for monitoring shellfish and shellfish growing water quality in New Zealand. © 2019 Elsevier Ltd Citation statistics:
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/121929
Appears in Collections: 气候变化事实与影响
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作者单位: Institute of Environmental Science and Research Ltd (ESR), Porirua, 5240, New Zealand; CSIRO Land and Water, Ecosciences PrecinctQueensland 4102, Australia; Institute of Environmental Science and Research Ltd (ESR), Christchurch, 8041, New Zealand
Recommended Citation:
Gyawali P.,Croucher D.,Ahmed W.,et al. Evaluation of pepper mild mottle virus as an indicator of human faecal pollution in shellfish and growing waters[J]. Water Research,2019-01-01,154