DOI: 10.1016/j.watres.2019.01.034
Scopus记录号: 2-s2.0-85061342769
论文题名: Prevalence, levels and seasonal variations of human enteric viruses in six major rivers in Alberta, Canada
作者: Pang X. ; Qiu Y. ; Gao T. ; Zurawell R. ; Neumann N.F. ; Craik S. ; Lee B.E.
刊名: Water Research
ISSN: 431354
出版年: 2019
起始页码: 349
结束页码: 356
语种: 英语
英文关键词: Cell culture
; Contamination
; Enteric viruses
; Human and livestock
; Quantitative PCR
; Surface water
Scopus关键词: Agriculture
; Cell culture
; Contamination
; Polymerase chain reaction
; Surface waters
; Urban growth
; Viruses
; Wastewater treatment
; Enteric virus
; Human and livestock
; Human enteric virus
; Potential impacts
; Quantitative PCR
; Seasonal variation
; Sequencing analysis
; Wastewater treatment plants
; Rivers
; river water
; surface water
; discharge
; disease prevalence
; livestock
; polymerase chain reaction
; river
; river pollution
; sampling
; seasonal variation
; virus
; wastewater treatment plant
; Adenoviridae
; Alberta
; Article
; Astroviridae
; enteric virus
; Enterovirus
; genotype
; human
; JC virus
; Norovirus
; prevalence
; priority journal
; Rotavirus
; Sapovirus
; seasonal variation
; viral contamination
; viral gastroenteritis
; virus detection
; waste water management
; water contamination
; Alberta
; Bow River
; Calgary
; Canada
; Edmonton
; Red Deer River
; Saskatchewan River
; Adenoviridae
; Astroviridae
; Cervus elaphus
; Enterovirus
; Human rotavirus A
; JC polyomavirus
; Norovirus
; Rotavirus
; Sapovirus
英文摘要: The prevalence and seasonal variation of 7 viruses in 6 major rivers in Alberta were assessed using a combination of qPCR, cell culture and integrated cell culture with qPCR (ICC-qPCR). Water samples were collected monthly from rivers at different sites upstream and downstream of major urban centers. Seven viruses including rotavirus, adenovirus, astrovirus, norovirus, sapovirus, JC virus and enterovirus, were detected in at least one of the water samples at each site using qPCR. Rotavirus was most common with concentration ranging from 2.3 to 4.5 log 10 genomic equivalent (GE) copies/L. Norovirus, sapovirus, astrovirus, adenoviruses and JC virus peaked during the winter (November to March). Viruses were most prevalent at the Bow River sampling site downstream of the City of Calgary, followed by the North Saskatchewan River site downstream of the City of Edmonton and the Red Deer River site downstream of the City of Red Deer. The detection rates and quantity of viruses had significant difference in the sampling sites between upstream and downstream of major urban centers (p < 0.001). 14% of the samples tested positive using viral culture indicating the presence of infectious viruses in river. Sequencing analysis identified human rotavirus in 75% of the samples collected from downstream versus 37% of the samples collected from upstream sites (p < 0.02). Multivariate binary regression showed that human activity in watersheds is a significant determinant of viruses in Alberta's Rivers. The discharge from wastewater treatment plants may be the possible sources of viral contamination. Seasonal coincidence of acute viral gastroenteritis outbreaks and monthly peak occurrence of enteric viruses in river water implies potential impact of waterborne viruses on human health. © 2019 Citation statistics:
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/121967
Appears in Collections: 气候变化事实与影响
There are no files associated with this item.
作者单位: Department of Laboratory Medicine and Pathology, University of Alberta, 116st & 85 Ave, Edmonton, AB T6G 2R3, Canada; Provincial Laboratory for Public Health, 8440-112st, Edmonton, AB T6G 2J2, Canada; Environmental Monitoring and Science Division, Alberta Environment and Parks, Edmonton, AB T5J 5C6, Canada; School of Public Health, University of Alberta, 1405-87 Ave, Edmonton, AB T6G 1C9, Canada; EPCOR Water, 9469 Rossdale Rd NW, Edmonton, AB T5K 2E9, Canada; Department of Paediatrics, University of Alberta, 1405-87 Ave, Edmonton, AB T6G 1C9, Canada
Recommended Citation:
Pang X.,Qiu Y.,Gao T.,et al. Prevalence, levels and seasonal variations of human enteric viruses in six major rivers in Alberta, Canada[J]. Water Research,2019-01-01