DOI: 10.1016/j.watres.2019.01.032
Scopus记录号: 2-s2.0-85061100536
论文题名: Mycobacterial metabolic characteristics in a water meter biofilm revealed by metagenomics and metatranscriptomics
作者: Zhu J. ; Liu R. ; Cao N. ; Yu J. ; Liu X. ; Yu Z.
刊名: Water Research
ISSN: 431354
出版年: 2019
起始页码: 315
结束页码: 323
语种: 英语
英文关键词: Biofilm
; Carbon fixation
; Drinking water distribution system
; Metabolic potential
; Mycobacteria
; Omics analysis
Scopus关键词: Carbon dioxide
; Gene expression
; Metabolism
; Organic carbon
; Photosynthesis
; Potable water
; Water distribution systems
; Water meters
; Carbon fixation
; Carbon fixation pathway
; Gene expression patterns
; Meta-cleavage pathway
; Metabolic characteristics
; Metabolic potential
; Mycobacteria
; Omics analysis
; Biofilms
; carbon dioxide
; catechol
; drinking water
; genomic DNA
; genomic RNA
; nitrogen
; nitrogenase
; organic carbon
; phosphate
; phosphorus
; RNA 16S
; virulence factor
; water
; bacterium
; biofilm
; carbon fixation
; drinking water
; gene expression
; genetic analysis
; genome
; genomics
; metabolism
; microbial community
; Article
; bacterial genome
; bacterial metabolism
; biofilm
; carbon dioxide fixation
; carbon fixation
; carbon source
; gene expression profiling
; gene sequence
; household
; metagenomics
; metatranscriptomics
; microbial community
; Mycobacterium
; Mycobacterium avium complex
; nonhuman
; phylogeny
; polymerase chain reaction
; priority journal
; transcriptomics
; water quality
; Bacteria (microorganisms)
; Corynebacterineae
; Mycobacterium avium complex
英文摘要: Mycobacteria represent one of the most persistent bacterial populations in drinking water distribution system (DWDS) biofilm communities; however, mycobacterial in situ metabolic profiles are largely unknown. In this study, the metabolic characteristics of mycobacteria in a household water meter biofilm were unveiled using a coupled metagenomic/metatranscriptomic approach. The water meter biofilm appeared to express nitrogenase genes (nifDKH) and a full complement of genes coding for several carbon-fixation pathways, especially the Calvin cycle, suggesting the CO 2 sequestration and dinitrogen fixation potential of the biofilm. These findings indicate that it may be difficult to prevent the formation of DWDS biofilms simply by controlling the availability of organic carbon or nitrogen. The composite genome of mycobacteria (CG-M) was reconstructed based on the obtained omics data. CG-M shared similar genome phylogeny and virulence-factor profiles with Mycobacterium avium complex, suggesting that population CG-M might represent a member of mycobacteria with pathogenicity. According to the gene expression patterns, population CG-M showed the metabolic potential to assimilate CO 2 via the Calvin cycle and/or anaplerotic reactions, and even to grow autotrophically with CO as the sole carbon and energy source. This suggests that organic carbon may not be a limiting factor for mycobacterial growth in DWDSs. Moreover, our results suggest that mycobacterial aromatic degradation is primarily achieved through the catechol meta-cleavage pathway, and biofilm mycobacteria could prefer phosphate as the phosphorus source. © 2019 Elsevier Ltd Citation statistics:
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/121970
Appears in Collections: 气候变化事实与影响
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作者单位: University of Chinese Academy of Sciences, Beijing, China; Institute of Microbiology, Chinese Academy of Sciences, Beijing, China; Beijing Waterworks Group, Beijing, China; Research Center for Eco-environmental Sciences, Chinese Academy of Sciences, Beijing, China
Recommended Citation:
Zhu J.,Liu R.,Cao N.,et al. Mycobacterial metabolic characteristics in a water meter biofilm revealed by metagenomics and metatranscriptomics[J]. Water Research,2019-01-01