DOI: 10.1016/j.watres.2018.12.060
Scopus记录号: 2-s2.0-85060254475
论文题名: Autonomous online measurement of Β-D-glucuronidase activity in surface water: is it suitable for rapid E. coli monitoring?
作者: Burnet J.-B. ; Dinh Q.T. ; Imbeault S. ; Servais P. ; Dorner S. ; Prévost M.
刊名: Water Research
ISSN: 431354
出版年: 2019
起始页码: 241
结束页码: 250
语种: 英语
英文关键词: Enzymatic assays
; Escherichia coli
; Online monitoring
; Repeatability
; Robustness
; β-D-glucuronidase
Scopus关键词: Automation
; Escherichia coli
; Microfiltration
; Monitoring
; Polymerase chain reaction
; Potable water
; Robustness (control systems)
; Sewage
; Water quality
; Water supply
; Enzymatic assay
; Faecal indicator bacteria
; Glucuronidase
; Microbiological water quality
; Near-real-time monitoring
; Online monitoring
; Quantitative real time PCR
; Repeatability
; Surface waters
; beta glucuronidase
; drinking water
; surface water
; bacterium
; bioassay
; enzyme
; enzyme activity
; Internet
; monitoring
; polymerase chain reaction
; substrate
; surface water
; water quality
; Article
; bacterial viability
; bacterium detection
; enzyme activity
; Escherichia coli
; intermethod comparison
; measurement accuracy
; monitoring
; nonhuman
; priority journal
; quantitative analysis
; real time polymerase chain reaction
; sediment
; sewage
; technology
; water quality
; water supply
; Escherichia coli
英文摘要: Microbiological water quality is traditionally assessed using culture-based enumeration of faecal indicator bacteria such as Escherichia coli. Despite their relative ease of use, these methods require a minimal 18-24 h-incubation step before the results are obtained. This study aimed to assess the suitability of an autonomous online fluorescence-based technology measuring β-glucuronidase (GLUC) activity for rapid near-real time monitoring of E. coli in water. The analytical precision was determined and compared to an automated microbial detection system, two culture-based assays and quantitative real-time PCR (qPCR). Using replicate measurements of grab samples containing E. coli concentrations between 50 and 2330 CFU.100 mL −1 , the autonomous GLUC activity measurement technology displayed an average coefficient of variation (CV) of less than 5% that was 4–8-fold lower than other methods tested. Comparable precision was observed during online in situ monitoring of GLUC activity at a drinking water intake using three independent instruments. GLUC activity measurements were not affected by sewage or sediments at concentrations likely to be encountered during long-term monitoring. Furthermore, significant (p < 0.05) correlations were obtained between GLUC activity and the other assays including defined substrate technology (r = 0.77), membrane filtration (r = 0.73), qPCR (r = 0.55) and the automated microbial detection system (r = 0.50). This study is the first to thoroughly compare the analytical performance of rapid automated detection technologies to established culture and molecular-based methods. Results show that further research is required to correlate GLUC activity to the presence of viable E. coli as measured in terms of CFU.100 mL −1 . This would allow the use of autonomous online GLUC activity measurements for rapid E. coli monitoring in water supplies used for drinking water production and recreation. © 2019 Elsevier Ltd Citation statistics:
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/122003
Appears in Collections: 气候变化事实与影响
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作者单位: NSERC Industrial Chair on Drinking Water, Department of Civil, Geological, and Mining Engineering, Polytechnique Montreal, Montreal, Quebec H3C 3A7, Canada; Canada Research Chair in Source Water Protection, Department of Civil, Geological, and Mining Engineering, Polytechnique Montreal, Montreal, Quebec H3C 3A7, Canada; Service de l'Environnement, Ville de Laval, QC H7L 2R3, Canada; Écologie des Systèmes Aquatiques, Université Libre de Bruxelles, Campus de la Plaine, CP 221, Boulevard du Triomphe, Bruxelles, B-1050, Belgium
Recommended Citation:
Burnet J.-B.,Dinh Q.T.,Imbeault S.,et al. Autonomous online measurement of Β-D-glucuronidase activity in surface water: is it suitable for rapid E. coli monitoring?[J]. Water Research,2019-01-01