DOI: 10.1016/j.watres.2018.12.036
Scopus记录号: 2-s2.0-85059737247
论文题名: Microbial communities controlling methane and nutrient cycling in leach field soils
作者: Fernández-Baca C.P. ; Omar A.-E.H. ; Pollard J.T. ; Richardson R.E.
刊名: Water Research
ISSN: 431354
出版年: 2019
起始页码: 456
结束页码: 467
语种: 英语
英文关键词: Methane
; Methanogen
; Methanotrophs
; Onsite wastewater treatment (OWTS)
; Septic leach field system
; Wastewater
Scopus关键词: Air quality
; Biomarkers
; Floods
; Gas emissions
; Genes
; Greenhouse gases
; Leaching
; Methane
; Methanogens
; Nutrients
; RNA
; Septic tanks
; Sewage
; Wastewater
; Wastewater treatment
; Biogeochemical cycling
; Dynamic environments
; Methanotrophs
; Microbial communities
; Microbial community structures
; Microbial populations
; On-site wastewater treatment
; Septic leach field system
; Soils
; carbon
; methane
; nitrogen
; oxygen
; phosphorus
; RNA 16S
; air quality
; community structure
; flooding
; genetic analysis
; leaching
; methane
; methanotrophy
; microbial activity
; microbial community
; nutrient cycling
; pollutant removal
; sewage treatment
; soil analysis
; soil column
; air quality
; amplicon
; Article
; biogeochemical cycling
; carbon cycling
; flooding
; greenhouse gas
; leaching
; mcra gene
; methanotroph
; microbial community
; microbial gene
; nitrogen cycling
; nonhuman
; nutrient cycling
; phosphorus cycle
; pmoa gene
; pollution
; priority journal
; sewage
; surface soil
; waste water
; water quality
英文摘要: Septic systems inherently rely on microbial communities in the septic tank and leach field to attenuate pollution from household sewage. Operating conditions of septic leach field systems, especially the degree of water saturation, are likely to impact microbial biogeochemical cycling, including carbon (C), nitrogen (N), and phosphorus (P), as well as greenhouse gas (GHG) emissions to the atmosphere. To study the impact of flooding on microbial methane (CH4) and nutrient cycling, two leach field soil columns were constructed. One system was operated as designed and the other was operated in both flooded and well-maintained conditions. CH4 emissions were significantly higher in flooded soils (with means between 0.047 and 0.33 g CH4 m−2 d−1) as compared to well-drained soils (means between −0.0025 and 0.004 g CH4 m−2 d−1). Subsurface CH4 profiles were also elevated under flooded conditions and peaked near the wastewater inlet. Gene abundances of mcrA, a biomarker for methanogens, were also greatest near the wastewater inlet. In contrast, gene abundances of pmoA, a biomarker for methanotrophs, were greatest in surface soils at the interface of CH4 produced subsurface and atmospheric oxygen. 16S rRNA, mcrA, and pmoA amplicon library sequencing revealed microbial community structure in the soil columns differed from that of the original soils and was driven largely by CH4 fluxes and soil VWC. Additionally, active microbial populations differed from those present at the gene level. Flooding did not appear to affect N or P removals in the soil columns (between 75 and 99% removal). COD removal was variable throughout the experiment, and was negatively impacted by flooding. Our study shows septic system leach field soils are dynamic environments where CH4 and nutrients are actively cycled by microbial populations. Our results suggest proper siting, installation, and routine maintenance of leach field systems is key to reducing the overall impact of these systems on water and air quality. © 2019 Elsevier Ltd Citation statistics:
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/122034
Appears in Collections: 气候变化事实与影响
There are no files associated with this item.
作者单位: Department of Civil and Environmental Engineering, Cornell University, 220 Hollister Hall, Ithaca, NY, United States; Department of Molecular Biology and Genetics, Cornell University, 107 Biotechnology Building, Ithaca, NY, United States
Recommended Citation:
Fernández-Baca C.P.,Omar A.-E.H.,Pollard J.T.,et al. Microbial communities controlling methane and nutrient cycling in leach field soils[J]. Water Research,2019-01-01