DOI: 10.1016/j.watres.2018.12.041
Scopus记录号: 2-s2.0-85059342658
论文题名: Novel ecological insights and functional roles during anaerobic digestion of saccharides unveiled by genome-centric metagenomics
作者: Zhu X. ; Campanaro S. ; Treu L. ; Kougias P.G. ; Angelidaki I.
刊名: Water Research
ISSN: 431354
出版年: 2019
起始页码: 271
结束页码: 279
语种: 英语
英文关键词: Energy conservation
; Metagenomics assembled genome
; Methane
; Microbial community
; Pathway reconstruction
; Syntrophic
Scopus关键词: Bacteria
; Ecology
; Electron transport properties
; Energy conservation
; Genes
; Metabolism
; Methane
; Physiology
; Substrates
; Continuous reactor operation
; Experimental conditions
; Heterogeneous substrates
; Metagenomics
; Microbial communities
; Pathway reconstruction
; Selective pressure
; Syntrophic
; Anaerobic digestion
; acetic acid
; carbohydrate
; genomic DNA
; glucose
; anaerobic digestion
; bioreactor
; energy conservation
; functional role
; genome
; genomics
; metabolism
; methane
; methanogenic bacterium
; microbial activity
; microbial community
; organic compound
; anaerobic digestion
; Article
; bacterial genome
; Clostridiaceae
; controlled study
; electron transport
; energy conservation
; genome centric metagenomic approach
; metagenome
; metagenomics
; Methanoculleus thermophilus
; microbial community
; mineralization
; nonhuman
; priority journal
; reactor operation
; species cultivation
; Clostridiaceae
; Methanoculleus thermophilus
英文摘要: In typical anaerobic digestion (AD) systems, the microbial functional assertion is hampered by synchronised versatile metabolism required for heterogeneous substrates degradation. Thus, the intricate methanogenic process from organic compounds remains an enigma after decades of empirical operation. In this study, simplified AD microbial communities were obtained with substrate specifications and continuous reactor operation. Genome-centric metagenomic approach was followed to holistically investigate the metabolic pathways of the AD and the microbial synergistic networks. In total, 63 metagenome assembled genomes (MAGs) were assembled from 8 metagenomes acquired in specific methanogenic niches. The metabolic pathways were reconstructed from the annotated genes and their dynamicity under experimental conditions. The results show that the methanogenic niches nourish unique metabolism beyond current knowledge acquired from cultivation-based methods. A novel glucose mineralization model without acetate formation was proposed and asserted in a pair of syntrophs: Clostridiaceae sp. and Methanoculleus thermophilus. Moreover, the catabolic pathway was elucidated in uncharacterized syntrophic acetate oxidizers, Synergistaceae spp. A remarkable evolutionary insight is the discovery that electron transport and energy conservation mechanisms impose selective pressure on syntrophic partners. Overall, the functional roles of the individual microbes tightly rely on the catabolic pathways and cannot always be physiologically defined in accordance with conventional four-step AD concept. The substrate-specific systems provided a traceable microbial community to dissecting the AD process. The genome-centric metagenomics successfully constructed genomes of microbes that have not been previously isolated and illustrated metabolic pathways that beyond the current knowledge of AD process. This study provides new perspectives to unravel the AD microbial ecology and suggests more attention should be paid on uncharacterized metabolism specifically harboured by AD microbial communities. © 2018 Elsevier Ltd Citation statistics:
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/122052
Appears in Collections: 气候变化事实与影响
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作者单位: Department of Environmental Engineering, Technical University of Denmark, Kgs. Lyngby, DK-2800, Denmark; Department of Biology, University of Padova, Via U. Bassi 58/b, Padova, 35121, Italy
Recommended Citation:
Zhu X.,Campanaro S.,Treu L.,et al. Novel ecological insights and functional roles during anaerobic digestion of saccharides unveiled by genome-centric metagenomics[J]. Water Research,2019-01-01