DOI: 10.1016/j.watres.2018.11.013
Scopus记录号: 2-s2.0-85056754704
论文题名: Microbiome of drinking water: A full-scale spatio-temporal study to monitor water quality in the Paris distribution system
作者: Perrin Y. ; Bouchon D. ; Delafont V. ; Moulin L. ; Héchard Y.
刊名: Water Research
ISSN: 431354
出版年: 2019
起始页码: 375
结束页码: 385
语种: 英语
英文关键词: 16S
; Bacteria
; Drinking water
; Ecology
; Microbiome
Scopus关键词: Bacteria
; Ecology
; Floods
; Genes
; Importance sampling
; RNA
; Throughput
; Water distribution systems
; Water quality
; Conventional methods
; Distribution systems
; Drinking water networks
; High-throughput sequencing
; Microbiological water quality
; Microbiome
; Physico - chemical parameters
; Spatio-temporal study
; Potable water
; drinking water
; RNA 16S
; bacterium
; comparative study
; drinking water
; microbial activity
; microbial ecology
; microorganism
; physicochemical property
; spatial distribution
; spatiotemporal analysis
; water quality
; Alphaproteobacteria
; amplicon
; Article
; bacterium culture
; flooding
; France
; high throughput sequencing
; Hyphomicrobium
; microbial community
; microbiome
; nonhuman
; Phreatobacter
; physical chemistry
; plate count
; polymerase chain reaction
; priority journal
; spatiotemporal analysis
; water quality
; water sampling
; water supply
; France
; Ile de France
; Paris
; Ville de Paris
; Bacteria (microorganisms)
; Hyphomicrobium
英文摘要: The microbiological water quality of drinking water distribution system (DWDS) is of primary importance for human health. High-throughput sequencing has gained more and more attention in the last decade to describe this microbial diversity in water networks. However, there are few studies describing this approach on large drinking water distribution systems and for extended periods of time. To fill this gap and observe the potential subtle variation in microbiota of a water network through time and space, we aimed to apply high-throughput sequencing of the 16S rRNA gene approach to characterize bacterial communities of the Paris’ DWDS over a one-year period. In this study, the Paris network, composed of four different DWDSs, was sampled at 31 sites, each month for one year. The sampling campaign was one of the largest described so far (n = 368) and the importance of key spatio-temporal and physico-chemical parameters was investigated. Overall, 1321 taxa were identified within the Paris network, although only fifteen of them were found in high relative abundance (>1%) in all samples. Two genera, Phreatobacter and Hyphomicrobium were dominant. The whole bacterial diversity was not significantly affected between the four DWDSs (spatial parameter) and by physico-chemical parameters. However, the bacterial diversity was slightly modified over the one-year period (temporal parameter) as we were able to observe DWDS microbiome perturbations, presumably linked to a preceding flood event. Comparison of high-throughput sequencing of the 16S rRNA gene amplicons vs. cultivation-based techniques showed that only 1.8% of bacterial diversity was recovered through cultivation. High throughput sequencing has made it possible to monitor DWDS more accurately than conventional methods by describing the whole diversity and detecting slight fluctuations in bacterial communities. This method would be further used to supervise drinking water networks, to follow any perturbations due to internals events (such as treatments) or external events (such as flooding). © 2018 Elsevier Ltd Citation statistics:
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/122146
Appears in Collections: 气候变化事实与影响
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作者单位: Laboratoire Ecologie et Biologie des Interactions, Equipes Microbiologie de l'Eau, et., Ecologie, Evolution, Symbiose, Université de Poitiers, UMR CNRS 7267, Poitiers, F-86073, France; Eau de Paris, Direction de la Recherche et du Développement pour la Qualité de l'Eau, R&D Biologie, 33, Avenue Jean Jaurès, Ivry sur Seine, F-94200, France; PBS – Bât B36/B37, 1 rue Georges Bonnet, TSA51106, POITIERS Cedex 9, 86073, France
Recommended Citation:
Perrin Y.,Bouchon D.,Delafont V.,et al. Microbiome of drinking water: A full-scale spatio-temporal study to monitor water quality in the Paris distribution system[J]. Water Research,2019-01-01