DOI: 10.1016/j.watres.2018.11.019
Scopus记录号: 2-s2.0-85056707401
论文题名: Antibiotic-resistance gene transfer in antibiotic-resistance bacteria under different light irradiation: Implications from oxidative stress and gene expression
作者: Chen X. ; Yin H. ; Li G. ; Wang W. ; Wong P.K. ; Zhao H. ; An T.
刊名: Water Research
ISSN: 431354
出版年: 2019
起始页码: 282
结束页码: 291
语种: 英语
英文关键词: Antibiotic-resistance bacteria
; Antibiotic-resistance genes
; Conjugative transfer
; Light irradiation
; Stress response
Scopus关键词: Antibiotics
; Escherichia coli
; Gene transfer
; Health risks
; Irradiation
; Light
; Oxidative stress
; Physiological models
; Physiology
; Public risks
; Radiation
; Antibiotic resistance bacteria
; Antibiotic resistance genes
; Conjugative transfer
; Light irradiations
; Stress response
; Gene expression
; beta lactamase CTX M
; polymyxin
; antibiotic resistance
; bacterium
; biochemistry
; gene expression
; gene transfer
; irradiation
; light effect
; oxidative stress
; physiology
; antibiotic resistance
; Article
; bacterial cell
; bacterial gene
; bacterium culture
; coculture
; Escherichia coli
; Escherichia coli C 600
; Escherichia coli DH 5 alpha
; gene expression
; gene frequency
; gene targeting
; gene transfer
; light
; nonhuman
; oxidative stress
; priority journal
; radiation exposure
; sunlight
; ultraviolet radiation
; Bacteria (microorganisms)
英文摘要: Due to the significant public health risks, there is substantial scientific interest in the increasing abundance of antibiotic-resistance bacteria (ARB) and the spread of antibiotic-resistance genes (ARGs) in aquatic environments. To clearly understand the mechanism of ARG transfer, this study examined the conjugative transfer of genes encoding resistance to cephalosporin (bla CTX ) and polymyxin (mcr-1) from two antibiotic-resistant donor strains, namely E. coli DH5α (CTX) and E. coli DH5α (MCR), and to a streptomycin-resistant receptor strain (E. coli C600 (Sm)). Conjugative transfer was specifically studied under different light irradiation conditions including visible light (VL), simulated sunlight (SS) and ultraviolet light (UV 254nm ). Results show that the conjugative transfer frequency was not affected by VL irradiation, while it was slightly improved (2–10 fold) by SS irradiation and extremely accelerated (up to 100 fold) by UV irradiation. Furthermore, this study also explored the link between ARG transfer and stress conditions. This was done by studying physiological and biochemical changes; oxidative stress response; and functional gene expression of co-cultured AR-E. coli strains under stress conditions. When correlated with the transfer frequency results, we found that VL irradiation did not affect the physiological and biochemical characteristics of the bacteria, or induce oxidative stress and gene expression. For SS irradiation, oxidative stress occurred slowly, with a slight increase in the expression of target genes in the bacterial cells. In contrast, UV irradiation, rapidly inactivated the bacteria, the degree of oxidative stress was very severe and the expression of the target genes was markedly up-regulated. Our study could provide new insight into the underlying mechanisms and links between accelerated conjugative transfer and oxidative stress, as well as the altered expression of genes relevant to conjugation and other stress responses in bacterial cells. © 2018 Elsevier Ltd Citation statistics:
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/122152
Appears in Collections: 气候变化事实与影响
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作者单位: Guangzhou Key Laboratory Environmental Catalysis and Pollution Control, School of Environmental Science and Engineering, Institute of Environmental Health and Pollution Control, Guangdong University of Technology, Guangzhou, 510006, China; School of Life Sciences, The Chinese University of Hong Kong, Shatin, NT, Hong Kong; Centre for Clean Environment and Energy, Griffith School of Environment, Gold Coast Campus, Griffith UniversityQueensland 4222, Australia
Recommended Citation:
Chen X.,Yin H.,Li G.,et al. Antibiotic-resistance gene transfer in antibiotic-resistance bacteria under different light irradiation: Implications from oxidative stress and gene expression[J]. Water Research,2019-01-01