DOI: 10.1016/j.watres.2018.10.089
Scopus记录号: 2-s2.0-85056216178
论文题名: The QuantiPhage assay: A novel method for the rapid colorimetric detection of coliphages using cellulose pad materials
作者: Rames E. ; Macdonald J.
刊名: Water Research
ISSN: 431354
出版年: 2019
起始页码: 98
结束页码: 110
语种: 英语
英文关键词: Cellulose fibre pad
; Colorimetric
; F+ coliphage
; Faecal contamination
; Somatic coliphage
; Water quality
Scopus关键词: Algae
; Bacteriophages
; Cellulose
; Color
; Colorimetry
; Polysaccharides
; Potable water
; Surface waters
; Water management
; Water quality
; Bacterial indicators
; Cellulose fibres
; Coliphages
; Colorimetric
; Colorimetric detection
; Faecal contamination
; Lower detection limit
; Microbial water quality
; Assays
; agar
; cellulose
; surface water
; assessment method
; bioassay
; bioindicator
; calorimetry
; cellulose
; detection method
; numerical method
; pollution monitoring
; sampling
; virus
; wastewater treatment
; water quality
; Article
; bacterial cell
; coliphage
; colorimetry
; controlled study
; immobilized cell
; intermethod comparison
; limit of detection
; nonhuman
; plaque assay
; priority journal
; sensitivity and specificity
; viral contamination
; virus concentration
; waste water
; water contamination
; water quality
; water supply
; Algae
; Cellulose
; Colorimetry
; Drinking Water
; Polysaccharides
; Bacteria (microorganisms)
英文摘要: Assessment of viral contamination is essential for monitoring the microbial quality of water and protection of public health, as human virus presence is not accurately determined using bacterial indicators. Currently, the time required for conventional viral testing means that water contaminated with human pathogens may be used (e.g. for drinking, recreation or irrigation) days before results are available. Here we report a new rapid method for coliphage enumeration, the QuantiPhage (QP) assay. The novelty of the assay is the use of cellulose absorbent pad materials to support coliphage growth and colorimetric detection, in place of agar that is used in the plaque assay. In addition to saving time associated with agar preparation and tempering, the QP assay enabled enumeration of somatic coliphages in 1.5–2 h and F+ coliphages in 2.5–3 h. The assays were highly sensitive, with a lower detection limit of 1 plaque forming unit (PFU) per mL where 1 mL sample volumes were analysed, and 1 PFU per 10 mL where 10 mL sample volumes were analysed. This is the first rapid culture assay to enable low numbers of coliphages to be reliably detected and to produce directly equivalent results to agar-based plaque assays. A novel gelatin-immobilisation method is also reported, that reduces time to prepare bacterial cells from ∼20 h to 40–60 min (depending on the assay format), and provides a ready to use form of cells, that is compatible with rapid detection and kit formats. When applied to analysis of somatic coliphages in wastewater samples and surface water samples, mean differences in results of the QP assay and the conventional plaque assay were not statistically significant (mean difference ≤ 0.15 log10 PFU/L and 0.5 PFU/10 mL respectively, P > 0.05). The QP is a valuable tool for assessing microbial water quality, which may assist in improving the management of water resources. © 2018 Elsevier Ltd Citation statistics:
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/122176
Appears in Collections: 气候变化事实与影响
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作者单位: Genecology Research Centre, School of Science and Engineering, University of the Sunshine Coast, Sippy Downs, Queensland, Australia; School of Natural Sciences, Griffith University, Nathan, Queensland, Australia; Future Biosolutions Pty Ltd, Buddina, Queensland, Australia; Division of Experimental Therapeutics, Department of Medicine, Columbia University, New York, United States
Recommended Citation:
Rames E.,Macdonald J.. The QuantiPhage assay: A novel method for the rapid colorimetric detection of coliphages using cellulose pad materials[J]. Water Research,2019-01-01