DOI: 10.1016/j.watres.2018.10.050
Scopus记录号: 2-s2.0-85055905177
论文题名: Ethyl tert-butyl ether (EtBE) degradation by an algal-bacterial culture obtained from contaminated groundwater
作者: van der Waals M.J. ; Plugge C. ; Meima-Franke M. ; de Waard P. ; Bodelier P.L.E. ; Smidt H. ; Gerritse J.
刊名: Water Research
ISSN: 431354
出版年: 2019
起始页码: 314
结束页码: 323
语种: 英语
英文关键词: 13C SIP
; Algal-bacterial culture
; Ethanol
; Micro-oxic EtBE degradation
; NMR
; TBA
Scopus关键词: Alcohols
; Bacteria
; Batch reactors
; Ethanol
; Gas chromatography
; Groundwater
; Groundwater pollution
; Health risks
; Nuclear magnetic resonance
; Nuclear magnetic resonance spectroscopy
; Oxygen supply
; Wastewater treatment
; Water treatment
; Algal-bacterial cultures
; Bacterial consortium
; Contaminated groundwater
; Degradation pathways
; Ethyl tert-butyl ether
; Microbial communities
; Stable-isotope probing
; Tert-butyl alcohols
; Biodegradation
; 2 hydroxyisobutyryl coenzyme A mutase
; alcohol
; carbon dioxide
; cytochrome P450
; ethyl tert butyl ether
; ground water
; methylmalonyl coenzyme A mutase
; mutase
; tert butyl alcohol
; unclassified drug
; unspecific monooxygenase
; alcohol
; alga
; bacterium
; biodegradation
; DNA
; ethanol
; ether
; metabolite
; microbial community
; nuclear magnetic resonance
; oxic conditions
; wastewater
; algal cell culture
; algal growth
; Article
; bacterium culture
; Blastocatellaceae
; carbon nuclear magnetic resonance
; Chlorella
; Comamonadaceae
; degradation
; gas chromatography
; Halomonadaceae
; Hyphomicrobiaceae
; microbial community
; Nocardiaceae
; nonhuman
; Oxalobacteraceae
; priority journal
; Rhodocyclaceae
; Scenedesmus
; Shewanellaceae
; Sphingomonadaceae
; water contamination
; water treatment
; algae
; Bacteria (microorganisms)
; Comamonadaceae
; Halomonadaceae
; Hyphomicrobiaceae
; Nocardiaceae
; Oxalobacteraceae
; Rhodocyclaceae
; Scenedesmus
; Shewanellaceae
; Sphingomonadaceae
英文摘要: EtBE is a fuel oxygenate that is synthesized from (bio)ethanol and fossil-based isobutylene, and replaces the fossil-based MtBE. Biodegradation of EtBE to harmless metabolites or end products can reduce the environmental and human health risks after accidental release. In this study, an algal-bacterial culture enriched from contaminated groundwater was used to (i) assess the potential for EtBE degradation, (ii) resolve the EtBE degradation pathway and (iii) characterize the phylogenetic composition of the bacterial community involved in EtBE degradation in contaminated groundwater. In an unamended microcosm, algal growth was observed after eight weeks when exposed to a day-night light cycle. In the fed-batch reactor, oxygen produced by the algae Scenedesmus and Chlorella was used by bacteria to degrade 50 μM EtBE replenishments with a cumulative total of 1250 μM in a day/night cycle (650 lux), over a period of 913 days. The microbial community in the fed-batch reactor degraded EtBE, using a P450 monooxygenase and 2-hydroxyisobutyryl-CoA mutase, to tert-butyl alcohol (TBA), ethanol and CO2 as determined using 13C nuclear magnetic resonance spectroscopy (NMR) and gas chromatography. Stable isotope probing (SIP) with 13C6 labeled EtBE in a fed-batch vessel showed no significant difference in community profiles of the 13C and 12C enriched DNA fractions, with representatives of the families Halomonadaceae, Shewanellaceae, Rhodocyclaceae, Oxalobacteraceae, Comamonadaceae, Sphingomonadaceae, Hyphomicrobiaceae, Candidatus Moranbacteria, Omnitrophica, Anaerolineaceae, Nocardiaceae, and Blastocatellaceae. This is the first study describing micro-oxic degradation of EtBE by an algal-bacterial culture. This algal-bacterial culture has advantages compared with conventional aerobic treatments: (i) a lower risk of EtBE evaporation and (ii) no need for external oxygen supply in the presence of light. This study provides novel leads towards future possibilities to implement algal-bacterial consortia in field-scale groundwater or wastewater treatment. © 2018 Elsevier Ltd Citation statistics:
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/122196
Appears in Collections: 气候变化事实与影响
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作者单位: Deltares, Subsurface and Groundwater Systems, Daltonlaan 600, Utrecht, BK 3584, Netherlands; Wageningen University & Research, Laboratory of Microbiology, Stippeneng 4, Wageningen, WE 6708, Netherlands; The Netherlands Institute of Ecology (NIOO-KNAW), Department of Microbial Ecology, Droevendaalsesteeg 10, Wageningen, PB 6708, Netherlands; Wageningen University & Research, BioNanoTechnology, Bornse Weilanden 9, Wageningen, EK 6700, Netherlands
Recommended Citation:
van der Waals M.J.,Plugge C.,Meima-Franke M.,et al. Ethyl tert-butyl ether (EtBE) degradation by an algal-bacterial culture obtained from contaminated groundwater[J]. Water Research,2019-01-01