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In the Anthropocene era, ecosystem productivity decreasing due to Global temperatures increasing and the biodiversity loss. Thus, are imperative that natural areas be explored under sustainable precepts, respecting and stimulating, the resilience and resistance of the ecosystems. To examine the relation of sustainable companies and the ecosystem services, we investigated all companies listed in the Corporate Sustainability Index (ISE) of Brazilian stock exchange (B3), considered as having as best sustainable practices of the Brazilian market. We analyzed if corporations dependently of provisioned ecosystem services recognizes the importance of sustainable practices for restocking the ecosystem services of natural areas that they explored. Data were collected reading socio-environmental reports. The LIFE certification methodology (Lasting Initiative for Earth) was used as guide-reading, making possible qualify and quantify the externalities and mitigations of each company. From seven companies examined, six not developed sufficient actions to mitigate their externalities. One company, from the forest sector, mitigated 40% of entire externalities calculated for all companies. Therefore, introduce biodiversity conservation actions in management systems contributes to achieving sustainable principles and the environmental conscious exploration. Additionally, the LIFE methodology can be used to improve sustainable policies of companies, mitigating their externalities, and approximating companies to achieve more easily the Paris Agreement through recompose the ecosystem services. (C) 2019 Elsevier Ltd. All rights reserved.