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globalchange > 气候减缓与适应

DOI:	10.1016/j.watres.2015.09.015
论文题名:	Molecular detection of Toxoplasma gondii in water samples from Scotland and a comparison between the 529bp real-time PCR and ITS1 nested PCR
作者:	Beth Wells^a; ; ; Hannah Shaw^a; ; Giles Innocent^b; ; Stefano Guido^a; ; Emily Hotchkiss^a; ; Maria Parigi^a; ^c; ; Marieke Opsteegh^a; ^d; ; James Green^e; ; Simon Gillespie^e; ; Elisabeth A. Innes^a; ; Frank Katzer^a;
刊名:	water Research
ISSN:	0043-1354
出版年:	2015
卷:	Volume 87, 页码:Pages 175-181
语种:	英语
英文关键词:	Toxoplasma gondii ; Waterborne transmission ; Public health ; Molecular detection
英文摘要:	Waterborne transmission of Toxoplasma gondii is a potential public health risk and there are currently no agreed optimised methods for the recovery, processing and detection of T.gondii oocysts in water samples. In this study modified methods of T.gondii oocyst recovery and DNA extraction were applied to 1427 samples collected from 147 public water supplies throughout Scotland. T.gondii DNA was detected, using real time PCR (qPCR) targeting the 529bp repeat element, in 8.79% of interpretable samples (124 out of 1411 samples). The samples which were positive for T.gondii DNA originated from a third of the sampled water sources. The samples which were positive by qPCR and some of the negative samples were reanalysed using ITS1 nested PCR (nPCR) and results compared. The 529bp qPCR was the more sensitive technique and a full analysis of assay performance, by Bayesian analysis using a Markov Chain Monte Carlo method, was completed which demonstrated the efficacy of this method for the detection of T.gondii in water samples.
URL:	http://www.sciencedirect.com/science/article/pii/S004313541530227X
Citation statistics:
资源类型:	期刊论文
标识符:	http://119.78.100.158/handle/2HF3EXSE/12950
Appears in Collections:	气候减缓与适应气候变化与战略

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作者单位:

^a Moredun Research Institute, Pentlands Science Park, Penicuik, Midlothian, EH26 0PZ, Scotland, United Kingdomid^b Biomathematics and Statistics Scotland (BioSS), JCMB, King's Buildings, Peter Guthrie Tait Road, Edinburgh, EH9 3FD, Scotland, United Kingdomid="aff3">^c School of Agriculture and Veterinary Medicine, University of Bologna, Italyid="aff4">^d National Institute for Public Health and the Environment (RIVM), P.O. Box 1, 3720 BA, Bilthoven, The Netherlandsid="aff5">^e Scottish Water, Juniper House, Heriot Watt Research Centre, Edinburgh, EH14 4AP, United Kingdom

Recommended Citation:

Beth Wells^a^,^,,et al. Molecular detection of Toxoplasma gondii in water samples from Scotland and a comparison between the 529bp real-time PCR and ITS1 nested PCR[J]. water Research,2015-01-01,Volume 87

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