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This study focuses on analysis of hydrological model parameter uncertainty at varying sub-basin spatial scales. It was found that the variation in sub-basin spatial scale had little influence on the entire flow simulations. However, the different sub-basin spatial scales had a significant impact on the reproduction of the flow quantiles. The coarser sub-basin spatial scale provided a better coverage of most prediction uncertainty in observations. However, the finer sub-basin spatial scale produced the best single simulation output closer to the observations. In general, the optimal sub-basin spatial scales (ratio to the entire watershed size) in the two test watersheds were found to be in the ranges 14-19% and 2-4% for good simulation of high and low flows, respectively. It is therefore worthwhile to put more effort into reproducing different flow quantiles by investigating an appropriate sub-basin spatial scale.