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Natural grasslands are being progressively degraded around the world due to human-induced action (e.g., overgrazing), but there is neither a widely accepted conceptual framework to approach degradation studies nor a clear definition of what grassland degradation is. Most of the drivers, processes, and consequences related to grassland degradation are widespread and are usually separately quoted in the literature. In this paper, we propose a comprehensive framework with different conceptual categories, for monitoring grassland degradation, and a new definition based on current ones. We provide a conceptual update of grassland degradation based on a literature review and an expert survey, focused on the Rio de la Plata grasslands (RPG). We identified drivers as external forces or changes that cause degradation; processes as measurable changes in grasslands conditions that can be evaluated using indicators; and consequences as the impacts or results of the process of grassland degradation. We expect that this conceptual framework will contribute to monitoring programs, to support management decisions, to design conservation measures, and to communicate the importance of grasslands conservation and the different concepts involved. Particularly for RPG, we expect that this paper will contribute to promote sustainable management practices in this important and often neglected ecosystem.