Groundwater, an essential resource, is likely to change with global warming because of changes in the CO2 levels, temperature and precipitation. Here, we combine water isotope geochemistry with climate modelling to examine future groundwater recharge in southwest Ohio, USA. We first establish the stable isotope profiles of oxygen and deuterium in precipitation and groundwater. We then use an isotope mass balance model to determine seasonal groundwater recharge from precipitation. Climate model output is used to project future changes in precipitation and its seasonal distribution under medium and high climate change scenarios. Finally, these results are combined to examine future changes in groundwater recharge. We find that 76% of the groundwater recharge occurs in the cool season. Climate models project precipitation increase in the cool season and decrease in the warm season. The total groundwater recharge is expected to increase by 3.2% (8.8%) under the medium (high) climate change scenarios.