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In this study, greenhouse gas (GHG) differences due to the application of biomass content are compared at a sewage sludge incinerator. The result of the comparison shows that the differences between the methods of GHG emission estimation based on biomass fraction analysis (sewage sludge analysis and sewage sludge flue gas analysis) were not substantial. On the other hand, the GHG emission estimated from the method in this study showed a difference of 8-9 ton CO(2)eq/day from the currently used method in Korea. This implies that the latter underestimates the GHG emissions because CO2 emission was not taken into account upon estimating the GHG emission from sewage sludge. Therefore, it has been determined that, from now on, emissions due to CO2 should be reflected in the estimation of GHG emission from sewage sludge.