This paper explores how medicinal plant knowledge of the Waorani (Ecuador) varies with socio-economic and demographic factors. Medicinal plant knowledge was compared at individual and community levels. Semi-structured interviews were performed with 56 informants (men N = 29, women N = 27) between 15 and 70 years old in five Waorani communities located within the Yasuni National Park and Waorani Ethnic Reserve. We found a positive correlation between an informant's medicinal plant knowledge and age, and a negative correlation between informant's medicinal plant knowledge and the years of schooling. Reasons behind these findings are thought to be in the rapid socio-cultural changes of the Waorani due to globalization processes. Increased accessibility to health centers and improved transportation infrastructure result in a loss of ethnobotanical knowledge.