We have established the stratigraphic sequence of DJH- 2 core, Dajiuhu Basin in Shennongjia Mountain. AMS dating and amino acid dating methods are applied to determine the age. Combined with climate stratigraphic correlation, the age of the core bottom is about275. 8 ka. According to the results of pollen analysis and sedimentary lithology, we have divided the vegetation succession and paleoclimate evolution into six stages since the late Middle Pleistocene. Stage one (275.8-245.0 ka): It was cold and dry, and coniferous forests and alpine meadows developed. Stage two (245.0-188.0 ka): It covered with temperate evergreen and deciduous broad-leaved forests. And it was warmer than stage one. Stage three (188.0-129.0 ka): The paleoclimate condition was similar to stage one, and its major vegetation shifted from coniferous forests to mountain meadow. Stage four (129.0-71.8 ka): The paleoclimate was warm and humid. And it was suitable for temperate broadleaf forests. Stage five (71.8-15.0 ka): The middle pollen subzone had the most of pollen concentration. The pollen belt reflected unstable climate but totally cold and wet. Stage six (15.0-1.0 ka): It was warm and humid to fit for the subtropical evergreen and deciduous broad-leaved forests. In comparison with oxygen isotope sequences of stalagmites (Sanbao caves) in East Asia and marine sediments (SPECMAP), the results show that the past vegetation changes of Shennongjia area responded sensitively to the climate changes. The variation of palynological assemblages followed climate changes, especially some species. The change of rhythm was similar to the global glacial-interglacial climate cycle. The climate change was induced by the ice volume from the high latitudes of the northern hemisphere. And it was also affected by solar radiation at low latitudes. The two factors played an important part in pollen concentration variation.