Based on seismic, logging, drilling cores and geochemical data, the sequence boundaries of the Kongdian Formation of Paleogene in the Jiyang Depression were systematically studied. On seismic profiles, the boundaries were featured by onlap, downlap, truncation, toplap and concordance. The drilling cores showed weathering crust and lithology-lithofacies transformation characteristics. The rock-electricity relation indicated different types of mutations. The trace elements also showed mutation characteristics. Four sequence boundaries were identified, including the 1st-order sequence interface TSB1, the 2nd-order sequence interface SSB1 and the 3rd-order sequence interfaces SB1 and SB2. As regional unconformity, the sequence interface TSB1 reflected ba-sin transformation. The sequence interface SSB1 was constructed ceremony conversion surface and showed basin rift stage. The sequence interfaces SB1 and SB2 were climate transformation surfaces and showed the cyclic chan-ges in climate factors. The sequence boundaries were significant for the studies of basin formation, climate change and screen fracture activity.